本文件规定了一种方法，通过测量密闭呼吸计中的需氧量来确定塑料材料（包括含有制剂添加剂的塑料材料）的有氧生物降解性程度。在实验室条件下，将测试材料在水性介质中暴露于来自活性污泥的接种物。 如果使用未适应的活性污泥作为接种物，则测试模拟在天然水环境中发生的生物降解过程；如果使用混合或预暴露接种物，则该方法用于研究测试材料的潜在生物降解性。 本文件中使用的条件不一定对应于允许发生最大生物降解的最佳条件，但本文件旨在确定塑料材料的潜在生物降解性或给出其在自然环境中的生物降解性的指示。该方法能够通过计算碳平衡（可选，见附件E）来改进对生物降解性的评估。 该方法适用于以下材料。 -天然和/或合成聚合物、共聚物或其混合物。 -含有添加剂如增塑剂、着色剂或其他化合物的塑料材料。 -水溶性聚合物。 -在测试条件下不抑制接种物中存在的微生物的材料。可以使用抑制对照或通过另一种合适的方法(参见例如ISO 8192[2])测定抑制作用。如果测试材料对接种物有抑制作用，则可以使用较低的测试浓度、另一种接种物或预先暴露的接种物。

This document specifies a method, by measuring the oxygen demand in a closed respirometer, for the determination of the degree of aerobic biodegradability of plastic materials, including those containing formulation additives. The test material is exposed in an aqueous medium under laboratory conditions to an inoculum from activated sludge. If an unadapted activated sludge is used as the inoculum, the test simulates the biodegradation processes which occur in a natural aqueous environment; if a mixed or pre-exposed inoculum is used, the method is used to investigate the potential biodegradability of a test material. The conditions used in this document do not necessarily correspond to the optimum conditions allowing maximum biodegradation to occur, but this document is designed to determine the potential biodegradability of plastic materials or give an indication of their biodegradability in natural environments. The method enables the assessment of the biodegradability to be improved by calculating a carbon balance (optional, see Annex E). The method applies to the following materials. — Natural and/or synthetic polymers, copolymers or mixtures thereof. — Plastic materials which contain additives such as plasticizers, colorants or other compounds. — Water-soluble polymers. — Materials which, under the test conditions, do not inhibit the microorganisms present in the inoculum. Inhibitory effects can be determined using an inhibition control or by another appropriate method (see, for example, ISO 8192^[2]). If the test material is inhibitory to the inoculum, a lower test concentration, another inoculum or a pre-exposed inoculum can be used.