本文件规定了一种慢性试验方法，用于评估土壤的栖息地功能，并通过以下方法确定土壤污染物和物质对尖叶虫繁殖的影响:？主要地消化吸收。本方法适用于未知质量的土壤和土壤材料，例如来自污染场地、改良土壤、修复后的土壤、工业、农业或其他受关注场地的土壤和废料（例如疏浚物、污水处理厂的城市污泥、合成材料或肥料，尤其是可能用于土地处置的材料）。繁殖（=幼体数量）是测试的测量参数。该测试反映了自然土壤（受污染场地土壤）中污染物混合物对代表其他ISO标准未涵盖的营养水平的物种的生物有效性。 本试验不打算取代蚯蚓（见ISO 11268-2）或弹尾目（见ISO 11267）繁殖试验，因为该物种不仅属于不同的营养类群，而且属于与通常使用的类群不同的分类类群（=螨；即蛛形纲动物）。 使用标准土壤（最好是规定的人工土壤基质）评估物质的影响。对于受污染的土壤，应在待测土壤和对照土壤中确定影响。根据研究的目的，对照和稀释基质（污染土壤的稀释系列）可以是与待测土壤（参考土壤）相当的未污染土壤，也可以是标准土壤（例如人工土壤）。 本文件提供了如何在温和条件下使用该方法测试样品（土壤或物质）的信息。 本文件不适用于空气/土壤分配系数大于1的物质，或25°C时蒸汽压力超过300 Pa的物质。 注:在试验期间，无法确保试验物质的稳定性。试验方法中没有规定监测受试物质的持久性。

This document specifies a chronic test method for evaluating the habitat function of soils and determining effects of soil contaminants and substances on the reproduction of Hypoaspis aculeifer by?mainly?alimentary uptake. This method is applicable to soils and soil materials of unknown quality, e.g. from contaminated sites, amended soils, soils after remediation, industrial, agricultural or other sites under concern and waste materials (e.g. dredged material, municipal sludge from a wastewater treatment plant, composed material, or manure, especially those for possible land disposal). The reproduction (= number of juveniles) is the measured parameter of the test. The test reflects the bioavailability of a mixture of contaminants in natural soils (contaminated site soils) to a species which represents a trophic level which is not covered by other ISO standards. This test is not intended to replace the earthworm (see ISO 11268-2) or Collembola (see ISO 11267) reproduction tests since this species belongs not only to a different trophic group but also a different taxonomic group (= mites; i.e. arachnids) than those used usually. Effects of substances are assessed using a standard soil, preferably a defined artificial soil substrate. For contaminated soils, the effects are determined in the soil to be tested and in a control soil. Depending on the objective of the study, the control and dilution substrate (dilution series of contaminated soil) are either an uncontaminated soil comparable to the soil to be tested (reference soil) or a standard soil (e.g. artificial soil). This document provides information on how to use this method for testing samples (soils or substances) under temperate conditions. This document is not applicable to substances for which the air/soil partition coefficient is greater than one, or to substances with vapour pressure exceeding 300 Pa at 25 °C. NOTE The stability of the test substance cannot be ensured over the test period. No provision is made in the test method for monitoring the persistence of the substance under test.