ISO 10253:2016规定了确定单细胞海藻骨骼细胞生长抑制的方法。和海藻中的物质和混合物，或环境水样（流出物，洗脱液等）中的三角褐指藻。 该方法可用于测试容易溶于水的物质，并且不会以任何其他方式从测试介质中显着降解或消除

ISO 10253:2016 specifies a method for the determination of the inhibition of growth of the unicellular marine algae Skeletonema sp. and Phaeodactylum tricornutum by substances and mixtures contained in sea water or by environmental water samples (effluents, elutriates, etc.). The method can be used for testing substances that are readily soluble in water and are not significantly degraded or eliminated in any other way from the test medium.