1.1 本指南提供了目前可用于评估天然或合成基质抗真菌活性的各种测试方法的信息。 1.2 实践本指南需要掌握微生物技术知识。 1.3 本标准并非旨在解决与其使用相关的所有安全问题（如有）。本标准的使用者有责任在使用前制定适当的安全、健康和环境实践，并确定监管限制的适用性。 1.4 本国际标准是根据世界贸易组织技术性贸易壁垒委员会发布的《关于制定国际标准、指南和建议的原则的决定》中确立的国际公认的标准化原则制定的。 ====意义和用途====== 4.1 众所周知，真菌会对各种消费品和建筑基底产生不良气味、污渍和过早的生物降解，包括纺织品、地毯、天花板瓷砖、石膏墙板、木材、增塑乙烯基和其他聚合物。 4.2 抗真菌活性通常为: 4.2.1 测定物品对真菌定殖的易感性， 4.2.2 抗真菌活性的测定（预防或延迟真菌定殖的定性测定），以及 4.2.3 杀真菌/杀孢子活性的测定（孢子杀灭的定量测定）。 4.3 在这些方法中，所需的表面检查程度各不相同，从肉眼检查到详细的显微镜评估。 4.4 本指南概述了已建立的方法及其适用性建议，并考虑到处理的底物类型或正在评估的抗真菌治疗类型。

1.1 This guide provides information on various test methods currently available to assess antifungal activity on natural or synthetic substrates. 1.2 Knowledge of microbiological techniques is required for the practice of this guide. 1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. 1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee. ====== Significance And Use ====== 4.1 Fungi are known to produce objectionable odors, stains, and premature biodeterioration of various consumer products and construction substrates including textiles, carpet, ceiling tile, gypsum wallboard, lumber, and plasticized vinyl and other polymers. 4.2 Antifungal activity is typically: 4.2.1 Determination of article susceptibility to fungal colonization, 4.2.2 Determination of fungistatic activity (qualitative determination of prevented or delayed fungal colonization), and 4.2.3 Determination of fungicidal/sporicidal activity (quantitative determination of spore kill). 4.3 The degree of required surface examination varies from gross visual examination to detailed microscopic assessment among these methods. 4.4 This guide provides an overview of established methods and suggestions for their applicability, with consideration to the type of substrate treated or the type of antifungal treatment being assessed.