1.1 本试验方法包括通过高效尺寸排斥色谱法（HPSEC）测定线性可溶性聚苯乙烯的平均分子量（MW）和分子量分布。该试验方法不是绝对的，需要使用商用窄分子量分布（MWD）聚苯乙烯标准进行校准。本试验方法适用于含有分子量成分的样品，其洗脱体积在聚苯乙烯标准规定的洗脱体积范围内（即分子量通常为2000至2 000 000 g·mol −1. ). 1.2 HPSEC与传统的尺寸排阻色谱SEC（也称为凝胶渗透色谱（GPC））不同，HPSEC系统每米的理论板数至少是传统SEC的十倍（见术语） D883 和实践 D3016 ). 2. HPSEC系统采用低容量液相色谱组件和填充相对较小（通常为3至20μm）微孔颗粒的色谱柱。需要用于数据采集和处理的高效液相色谱仪器和自动数据处理系统。 1.3 以国际单位制表示的数值应视为标准。 1.4 本标准并非旨在解决与其使用相关的所有安全问题（如有）。本标准的用户有责任在使用前制定适当的安全、健康和环境实践，并确定监管限制的适用性。 第节给出了具体的预防说明 9 . 注1: 没有已知的ISO等效于本标准。 1.5 本国际标准是根据世界贸易组织技术性贸易壁垒（TBT）委员会发布的《关于制定国际标准、指南和建议的原则的决定》中确立的国际公认标准化原则制定的。 ====意义和用途====== 5.1 通用设施- 分子量（MW）和分子量分布（MWD）是聚合物样品的基本特征。它们用于基础研究、加工或产品应用的各种相关性。例如，将观察到的MWD与根据聚合反应的假设动力学或机理预测的MWD进行比较。数值之间的差异将允许更改理论或实验设计。类似地，聚合物样品的强度、熔体流动和其他特性通常取决于分子量和分子量密度。分子量和分子量密度的测定用于聚合物的质量控制。 5.2 限制- 由于需要对所研究的聚合物类型进行特定校准，并且由于聚合物/溶剂/柱填料相互作用的特殊性质，本试验方法仅适用于聚苯乙烯和非聚苯乙烯- 应避免排斥效应。然而，该方法的许多原理已应用于生成其他聚合物系统的HPSEC方法，例如，使用通用校准原理。（参见实践 D3016 ).

1.1 This test method covers the determination of molecular weight (MW) averages and the distribution of molecular weights for linear, soluble polystyrene by high-performance size-exclusion chromatography (HPSEC). This test method is not absolute and requires the use of commercially available narrow molecular weight distribution (MWD) polystyrene standards for calibration. This test method is applicable for samples containing molecular weight components that have elution volumes falling within the elution volume range defined by polystyrene standards (that is, molecular weights generally from 2000 to 2 000 000 g·mol −1 ). 1.2 The HPSEC is differentiated from traditional size-exclusion chromatography SEC (also referred to as gel permeation chromatography (GPC)) in that the number of theoretical plates per metre with an HPSEC system is at least ten times greater than that for traditional SEC (see Terminology D883 and Practice D3016 ). 2 The HPSEC systems employ low-volume liquid chromatography components and columns packed with relatively small (generally 3 to 20 μm) microporous particles. High-performance liquid chromatography instrumentation and automated data handling systems for data acquisition and processing are required. 1.3 The values stated in SI units are to be regarded as the standard. 1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. Specific precautionary statements are given in Section 9 . Note 1: There is no known ISO equivalent to this standard. 1.5 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee. ====== Significance And Use ====== 5.1 General Utility— The molecular weight (MW) and molecular weight distribution (MWD) are fundamental characteristics of a polymer sample. They are used for a wide variety of correlations for fundamental studies, processing, or product applications. For example, the observed MWD is compared to one predicted from assumed kinetics or mechanisms for a polymerization reaction. Differences between the values will allow alteration of theory or experimental design. Similarly, the strength, melt flow, and other properties of a polymer sample usually are dependent on MW and MWD. Determinations of MW and MWD are used for quality control of polymers. 5.2 Limitations— Because of the need for specific calibration of the polymer type under study, and because of the specific nature of polymer/solvent/column-packing interactions, this test method is valid only for polystyrene and non-exclusion effects are to be avoided. However, many of the principles of the method have been applied in generating HPSEC methods for other polymer systems, for example, using the principles of universal calibration. (see Practice D3016 ).