ISO 16649-1:20 18规定了一种水平方法，用于在使用膜复苏并在44℃下在含有显色成分的固体培养基上孵育后，通过菌落计数技术计数β-葡萄糖醛酸酶阳性大肠杆菌，用于检测酶β-葡萄糖醛酸酶[9][10][13][14][17][18][19][20]。它适用于 -拟供人食用的产品， -用于喂养动物的产品， -食品生产和食品处理领域的环境样本，以及 -来自初级生产阶段的样品，如动物粪便、灰尘和拭子。

ISO 16649-1:2018 specifies a horizontal method for the enumeration of β-glucuronidase-positive Escherichia coli by colony-count technique after resuscitation using membranes and incubation at 44 °C on a solid medium containing a chromogenic ingredient for detection of the enzyme β-glucuronidase^{[9][10][13][14][17][18][19][20]}. It is applicable to - products intended for human consumption, - products intended for feeding animals, - environmental samples in the area of food production and food handling, and - samples from the primary production stage such as animal faeces, dust, and swabs.