方法1623在50L试剂水和PE样品中的隐孢子虫回收率分别为60%和62%，RSD为11%。在10 L基质峰中，平均回收率为42%，RSD为13%。结果表明，Filta Max filter从不同基质中回收率高，RSD低。为了简化野外采样过程，设计并测试了一种简单、方便、快速的采样装置。在19个地表水样本中，通过1623方法检测出7个隐孢子虫阳性，范围为每10升1到10个卵囊。在这7个阳性样本中，5个通过PCR检测也呈隐孢子虫阳性。通过RFLP在其中4个地表水样中鉴定了微小隐球菌牛株。一个样本同时含有C。 牛细小病毒株和鼠细小病毒株。方法1623阴性的12份样品经PCR均为阴性。结果表明，两种方法之间有高度一致性（95%）。结果证明，使用单过滤器（Filta Max）检测隐孢子虫的存在、确认隐孢子虫的存在、量化卵囊的数量以及鉴定地表水样中隐孢子虫的种类是可行的。包括5个参考文献、表格、图表。

The recoveries of Cryptosporidium using Method 1623 in 50 L of reagent water and PE samples were 60% and 62%, respectively with 11% RSD. In 10 L of matrix spikes, the mean recovery was 42% with 13% RSD. The results indicate that Filta-Max filter gave sufficient recovery from different matrices with a low RSD. To faciliate the sampling process in the field, a simple, convenient, and fast sampling apparatus was constructed and tested. Of 19 surface water samples, 7 were positive for Cryptosporidium by Method 1623 with a range of 1 to 10 oocysts per 10 L. Among these 7 positive samples, 5 were also positive for Cryptosporidium by PCR. C. parvum bovine strain were identified by the RFLP in 4 of these surface water samples. One sample contained both C. parvum bovine strain and C. muris. All 12 samples negative by Method 1623 were negative by PCR. The results demonstrated a high degree of agreement (95%) between the two methods. The results prove it is feasible to use single filter (Filta-Max) to detect the presence of Cryptosporidium, confirm that presence, quantify the numbers of oocysts, and identify the species of Cryptosporidium present in surface water samples. Includes 5 references, tables, figures.