本文件适用于被归类为PFC 1（A）和PFC 1（B）的施肥产品，或2019/1009[1]号法规PFC 7中的PFC 1（A）和PFC 1（B）成分。然而，本方法未对共混物进行验证。 本文件规定了在44°C下，在含有显色成分的固体培养基上进行菌落计数的技术，用于检测β-葡萄糖醛酸酶。该方法基于ISO 16649-2[4]。 不会检测到44°C下不生长的大肠杆菌菌株，尤其是β-葡萄糖醛酸酶阴性的菌株，例如大肠杆菌O157。由于某些肠杆菌科，特别是志贺氏菌和沙门氏菌，在44°C下也可以显示β-葡萄糖醛酸酶活性，因此检测到的微生物被推定为β-葡萄糖醛酸酶阳性大肠杆菌。 购买本文件时可获得的所有当前修订均包含在购买本文件中。

This document is applicable to fertilizing products, which are classified as PFC 1(A) and PFC 1(B) or the PFC 1(A) and PFC 1(B) component in PFC 7 of Regulation (EU) 2019/1009 [1]. However, the present method was not validated for blends. This document specifies a colony-count technique at 44 °C on a solid medium containing a chromogenic ingredient for the detection of the enzyme β-glucuronidase. The method is based on ISO 16649-2 [4]. Strains of Escherichia coli which do not grow at 44 °C and, in particular, those that are β-glucuronidase negative, such as Escherichia coli O157, will not be detected. Detected microorganisms are presumptively determined β-glucuronidase-positive Escherichia coli, since some Enterobacteriaceae, in particular Shigella and Salmonella, can also show β-glucuronidase activity at 44 °C.All current amendments available at time of purchase are included with the purchase of this document.