ISO 5667-16:20 17为进行生物测试时环境样品的取样、预处理、性能和评估提供了实用指南。给出了如何处理样品产生的生物测试问题和测试设计的适用性的信息。 它旨在通过描述成功地解决或规避例如水的生物测试的一些实验问题的方法来传达关于要采取的预防措施的实践经验。 主要处理与物质有关的问题，涉及环境样品（如废水样品）的取样和预处理，以进行生物测试。 本指南是关于生物体的生态毒理学试验（单物种生物试验；体内和体外）。就取样和样品制备而言，本文件中讨论的一些特征也适用于使用单细胞系统的生物测试（体外生物测定）和生物降解研究。还讨论了水溶性范围内物质的测试。 已尽可能参考现有的国际标准和准则。从发表的论文或口头交流中获取的信息也被利用。 ISO 5667-16:20 17适用于确定环境样品影响的生物测试，如处理过的公共和工业废水、地下水、淡水、水提取物（如渗滤液、洗脱液）、沉积物的孔隙水和整个沉积物。本文件也适用于化学物质。 ISO 5667-16:20 17不适用于水的细菌学检查。其他文件中描述了细菌学检查的适当方法（参见ISO 19458[17]）。

ISO 5667-16:2017 gives practical guidance on sampling, pre-treatment, performance and evaluation of environmental samples in the context of performing biological tests. Information is given on how to cope with the problems of biotesting arising from the sample and the suitability of the test design. It is intended to convey practical experience concerning precautions to be taken by describing methods successfully proven to solve or to circumvent some of the experimental problems of biotesting of, for example, waters. Primarily dealt with are substance-related problems concerning sampling and pre-treatment of environmental samples (e.g. waste water samples) for the performance of biotests. This guidance is on ecotoxicological testing with organisms (single-species biotests; in vivo and in vitro). Some features addressed in this document also apply to biotests using single-cell systems (in vitro bioassays) and biodegradation studies as far as sampling and sample preparations are concerned. Testing of substances in the water solubility range is also addressed. Reference has been made as far as possible to existing International Standards and guidelines. Information taken from published papers or oral communication has been utilized as well. ISO 5667-16:2017 is applicable to biological tests for determining the effect of environmental samples like treated communal and industrial waste water, groundwater, fresh water, aqueous extracts (e.g. leachates, eluates), pore water of sediments and whole sediments. This document is also applicable to chemical substances. ISO 5667-16:2017 is not applicable to bacteriological examination of water. Appropriate methods for bacteriological examination are described in other documents (see ISO 19458^[17]).