该方法包括对糖苷酶解或酸水解后释放的酸进行蒸汽蒸馏，然后通过在氨介质中用硝酸银溶液直接滴定馏出物并在碘化钾存在下测定所获得的酸的量，或通过在充分稀释的硝酸介质中和在铁（III）离子存在下用硫氰酸铵溶液反滴定过量的硝酸银溶液。附录描述了汞量法的测定。

The method consists in steam distillation of the acid liberated after enzymatic or acid hydrolysis of the glycosides, followed by determination of the quantity of acid obtained, either by direct titration in the distillate with silver nitrate solution in an ammoniacal medium and in the presence of potassium iodide, or by back titration of an excess of silver nitrate solution with ammonium thiocyanate solution, in a suffiently diluted nitric acid medium and in the presence of iron(III) ions. An annex describes a determination by the mercurimetric method.