Microbiology of the food chain - Methods for the detection of Anisakidae L3 larvae in fish and fishery products - Part 2: Artificial digestion method (ISO 23036-2:2021)
食物链微生物学鱼类和水产品中异尖线虫L3幼虫的检测方法第2部分:人工消化法
发布日期:
2021-05-12
实施日期:
2021-05-12
ISO 23036的这一部分规定了一种适用于检测海洋和溯河产卵鱼类中常见的异尖杉科L3幼虫的方法。该方法适用于鲜鱼和/或冷冻鱼、腌制、腌制或熏制等轻加工鱼制品,也适用于内脏器官,作为目测方案的确认方法。
人工消化法可以通过估计鱼肌肉组织中寄生虫的数量来量化寄生虫感染,当应用于新鲜鱼或轻加工的鱼制品(加工前从未冷冻)时,可以确定可能存在的异尖杉科L3的生存能力。
这种方法不允许确定检测到的寄生虫的种类或基因型,这种鉴定是通过形态学和/或分子方法进行的。
This document specifies a method for the detection of Anisakidae L3 larvae commonly found in marine and anadromous fishes. The method is applicable to fresh fish and/or frozen fish, as well as lightly processed fish products, such as marinated, salted or smoked. It is also suitable for visceral organs as a confirmatory method for a visual inspection scheme.
The artificial digestion method[4][5][6] is applicable to quantifying parasitic infections by estimating the number of parasites in the fish musculature and, when applied to fresh fish or lightly processed fish products (never frozen before processing), determining the viability of Anisakidae L3, which can be present.
This method does not apply to determining the species or genotype of detected parasites. Final identification is made by morphological and/or molecular methods.