Infant formula and adult nutritionals. Determination of β-carotene, lycopene and lutein by reversed-phase ultra-high performance liquid chromatography (RP-UHPLC)
婴儿配方奶粉和成人营养品 决定-反相超高效液相色谱法测定胡萝卜素、番茄红素和叶黄素
发布日期:
2020-07-06
本文件规定了一种定量测定食品中β-胡萝卜素和番茄红素的方法
固体(即粉末)或液体(即即食液体和液体)的婴儿配方奶粉和成人营养品
浓缩物)使用反相超高效液相色谱法(RP-UHPLC)形成
以及紫外可见检测。番茄红素的应用范围为1µg/100 g至1500µg/100 g
β-胡萝卜素的含量为1微克/100克到2250微克/100克。基于单个实验室的验证
检测限(LOD)为0.1µg/100 g,定量限(LOQ)为0.3µg/100 g
每种类胡萝卜素。购买本文件时可获得的所有当前修订均包含在购买本文件中。
This document specifies a method for the quantitative determination of β-carotene and lycopene in
infant formula and adult nutritionals in solid (i.e. powders) or liquid (i.e. ready-to-feed liquids and liquid
concentrates) forms using reversed-phase ultra-high performance liquid chromatography (RP-UHPLC)
and UV-visible detection. The application range runs from 1 µg/100 g to 1 500 µg/100 g for lycopene
and from 1 µg/100 g to 2 250 µg/100 g for β-carotene. Based on the single-laboratory validation, the
limit of detection (LOD) was 0,1 µg/100 g and the limit of quantification (LOQ) was 0,3 µg/100 g for
each carotenoid.All current amendments available at time of purchase are included with the purchase of this document.