1.1 本试验方法涵盖了在生物安全3级（BSL3）实验室处理纤维素擦拭布的标准方法，以检测并提供半定量估计 炭疽杆菌 非多孔表面取样后的污染。可进行采样以表征污染程度，或用于净化后的区域清理。 1.2 实验室程序应由受过BSL3微生物技术培训的人员在BSL3实验室中执行。 1.3 本试验方法适用于 B、 炭疽病 ，但可以与其他生物体一起使用。 1.4 实验室间研究是用中和缓冲液预湿的纤维素海绵擦拭布进行的。所有再现性、灵敏度和特异性数据均基于这些湿巾的性能。小组委员会于2019年进行了审查，并再次确认这些ILS数据有效。 1.5 单位- 以国际单位制表示的数值应视为标准值。国际单位制后括号中给出的值仅供参考，不被视为标准值。 1.6 本标准并非旨在解决与其使用相关的所有安全问题（如有）。本标准的用户有责任在使用前制定适当的安全、健康和环境实践，并确定监管限制的适用性。 1.7 本国际标准是根据世界贸易组织技术性贸易壁垒（TBT）委员会发布的《关于制定国际标准、指南和建议的原则的决定》中确立的国际公认标准化原则制定的。 ====意义和用途====== 5.1 本程序描述了在生物安全3级实验室处理纤维素擦拭布的标准化方法，以检测并提供半成品- 定量估计 B、 炭疽病 非多孔表面取样后的污染。可进行采样，以确定污染程度或净化后清除区域。

1.1 This test method covers a standardized method of processing cellulose wipes in a biosafety level 3 (BSL3) laboratory in order to detect and provide a semi-quantitative estimate of Bacillus anthracis contamination after sampling of non-porous surfaces. Sampling may be conducted to characterize the extent of the contamination, or for area clearance after decontamination. 1.2 The laboratory procedures should be performed in a BSL3 laboratory by those trained for BSL3 microbiological techniques. 1.3 This test method is specific to B. anthracis , but could be adapted for use with other organisms. 1.4 The interlaboratory study was conducted with cellulose sponge wipes pre-moistened with neutralizing buffer. All reproducibility, sensitivity, and specificity data are based on the performance of these wipes. A review was conducted by subcommittee in 2019, and re-confirmed these ILS data are valid. 1.5 Units— The values stated in SI units are to be regarded as standard. The values given in parentheses after SI units are provided for information only and are not considered standard. 1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. 1.7 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee. ====== Significance And Use ====== 5.1 This procedure describes a standardized method of processing cellulose wipes in a biosafety level 3 laboratory in order to detect and provide a semi-quantitative estimate of B. anthracis contamination after sampling of non-porous surfaces. Sampling may be conducted to characterize the extent of contamination or for clearance of an area after decontamination.