过去十年中，研究人员和水务公司在广泛的采样计划下收集了用于隐孢子虫和隐孢子虫分析的样本，其中包括由大型水务公司强制进行定期采样的信息收集规则。然而，还没有对样本采集设计进行系统研究，以确保此类采样计划收集的数据代表了流域。该项目旨在收集一组可靠的数据，以评估六个具有高度特征的水源水中病原体的时间变异性，并检查在各种流域条件下最强烈影响这种变异性的因素； 对隐孢子虫计数的两种最具预测性的方法进行详细评估，并开发一个关于活卵囊和传染性卵囊的数据库；此外，评估原生动物与参数之间的关系，如总大肠菌群和粪便大肠菌群、大肠杆菌、产气荚膜梭菌、硬度、pH值、浊度、温度、流速和其他现场特定参数。包括8个参考文献、表格、图表。

Researchers and water utilities over the past decade have collected samples forGiardia and Cryptosporidium analysis under a wide scenario of sampling schedulesthat include the Information Collection Rule-mandated regular sampling by largerutilities. However, there has not been a systematic study of sample collectiondesigns to ensure that such sampling programs are collecting data representativeof the watershed. This project was designed to collect a robust set of data to:evaluate the temporal variability of pathogens in six highly characterized sourcewaters, and examine the factors that most strongly affect this variability for arange of watershed conditions; provide a detailed evaluation of the two mostpromising methods for enumerating Cryptosporidium and develop a database forviable and infectious oocysts; and, evaluate relationships between protozoa andparameters such as total and fecal coliforms, E. coli, Clostridium perfringens,hardness, pH, turbidity, temperature, flow rate, and other site-specificparameters. Includes 8 references, tables, figures.