1.1 这种做法用于从城市废水污泥中回收病毒，有利于人类来源的肠道病毒。 1.2 这两种程序都适用于原污泥、消化污泥和脱水污泥。 章节 程序A-吸附 7 到 11 程序B-超声处理 12 到 16 1.3 该实践在标准化污泥上进行了测试，如中所述 11.1 用户有责任确保本实践对于未经测试的基质的有效性。 1.4 以SI单位表示的值将被视为标准值。本标准不包括其他计量单位。 1.5 本标准并不旨在解决与其使用相关的所有安全性问题（如果有）。本标准的使用者有责任在使用前建立适当的安全、健康和环境实践并确定法规限制的适用性。1.6 只有经过充分培训的人员才应被允许执行这些程序，并应使用美国公共卫生局、疾病控制中心， 2 用于处理潜在危险生物有机体的工作。 1.7 本国际标准是根据世界贸易组织技术性贸易壁垒（TBT）委员会发布的《关于制定国际标准、指南和建议的原则的决定》中确立的国际公认的标准化原则制定的。 ======意义和用途====== 4.1 尽管许多实验室目前正在从污泥中分离病毒，但由于缺乏标准测试方法，无法对产生的数据进行有效比较。

1.1 This practice is used for the recovery of viruses from municipal wastewater sludges and favors the enteroviruses of human origin. 1.2 Both procedures are applicable to raw, digested, and dewatered sludges. Sections Procedure A—Adsorption 7 to 11 Procedure B—Sonication 12 to 16 1.3 This practice was tested on standardized sludges as described in 11.1 . It is the user's responsibility to ensure the validity of this practice for untested matrices. 1.4 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard. 1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. 1.6 Only adequately trained personnel should be allowed to perform these procedures and should use safety precautions recommended by the U.S. Public Health Service, Center for Disease Control, 2 for work with potentially hazardous biological organisms. 1.7 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee. ====== Significance And Use ====== 4.1 Although many laboratories are presently isolating viruses from sludge, a valid comparison of data generated has not been possible because of the lack of a standard test method(s).