该方法适用于棉籽和棉籽饼。该方法包括在3-氨基丙烷-1-醇存在下提取化合物，使用2-丙醇和己烷混合物测定游离棉酚，或使用二甲基甲酰胺测定总棉酚。使用苯胺转化为棉酚二苯胺，并在最大吸光度波长（435至445 nm之间）处测量吸光度。

The method is applicable to cottonseed and cottonseed meals and cakes. The method consists in extracting of the compound in the presence of 3-aminopropane-1-ol, either with a mexture of 2-propanol and hexane for the determination of free gossypol or with dimethylformamide for the determination of total gossypol. Conversion to gossypol-dianiline using aniline, and measurement of the absorbance at the wavelength of maximum absorbance (between 435 and 445 nm).