1.1本规程阐述了获得精确双谱光度数据以计算荧光样品颜色所需的仪器测量要求、校准程序和材料标准。 1.2本规程列出了在特定方法、规程或规范中需要进行双谱光度测量时必须指定的参数。 1.3本规程特别适用于双谱仪，其产生光度定量双谱数据作为输出，有助于表征外观，与荧光分光光度计相反，荧光分光光度计产生仪器相关双谱光度数据作为输出，有助于化学分析。 1.4本规程的范围仅限于讨论反射几何形状下的物体颜色测量；它不包括在透射几何形状下对试样进行类似表征的规定。 本标准可能涉及危险材料、操作和设备。本标准并非旨在解决与其使用相关的所有安全问题（如有）。本标准的用户有责任在使用前制定适当的安全和健康实践，并确定监管限制的适用性。 ====意义和用途====== 双谱或双单色仪法是确定一般光源的确定方法- 荧光样品的辐射传输特性 (2) . Donaldson辐射因子是与仪器和光源无关的样本光度特性，可用于计算任何所需光源和观察者的颜色。该方法的优点是，它提供了样本辐射传输特性的全面表征，而不存在与源模拟和各种近似方法相关的不精确性。 本规程提供了选择用于提供所需精度数据的双谱仪工作参数的程序。它还规定了通过材料标准进行仪器校准，以及选择合适的样本以获得测量精度。

1.1 This practice addresses the instrumental measurement requirements, calibration procedures, and material standards needed for obtaining precise bispectral photometric data for computing the colors of fluorescent specimens. 1.2 This practice lists the parameters that must be specified when bispectral photometric measurements are required in specific methods, practices, or specifications. 1.3 This practice applies specifically to bispectrometers, which produce photometrically quantitative bispectral data as output, useful for the characterization of appearance, as opposed to spectrofluorimeters, which produce instrument-dependent bispectral photometric data as output, useful for the purpose of chemical analysis. 1.4 The scope of this practice is limited to the discussion of object-color measurement under reflection geometries; it does not include provisions for the analogous characterization of specimens under transmission geometries. This standard may involve hazardous materials, operations, and equipment. This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. ====== Significance And Use ====== The bispectral or two-monochromator method is the definitive method for the determination of the general (illuminant-independent) radiation-transfer properties of fluorescent specimens (2) . The Donaldson radiance factor is an instrument- and illuminant-independent photometric property of the specimen, and can be used to calculate its color for any desired illuminant and observer. The advantage of this method is that it provides a comprehensive characterization of the specimen's radiation-transfer properties, without the inaccuracies associated with source simulation and various methods of approximation. This practice provides a procedure for selecting the operating parameters of bispectrometers used for providing data of the desired precision. It also provides for instrument calibration by means of material standards, and for selection of suitable specimens for obtaining precision in the measurements.