本研究的目的是开发一种隐孢子虫分析方法，该方法操作简单，成本低，并提供可重复的结果，不依赖于特定操作员的技能或分析实验室的能力。这项研究的重点是使用一种新型泡沫过滤器Filta Max（TM），该过滤器可用于过滤成品水和原水。过滤器非常紧凑，直径5.5厘米，深度5厘米，适合10厘米深的外壳，因此非常适合在现场使用。过滤洗脱液可在10分钟内浓缩至约20毫升。不需要离心。然后，使用泡沫过滤器生成的浓缩物接受新型免疫磁性分离（IMS）系统，无需离心和再悬浮。新型IMS技术（Puri）- Max[TM]）依赖于样品（加上顺磁珠）通过位于磁场中的小捕获相的重复循环。卵囊可以通过抗体包被的顺磁珠捕获在相上，多余的样本被洗掉。然后将卵囊从顺磁性阶段移除，即可简单地恢复卵囊。数据显示，使用Filta Max/Puri Max组合，可从含有100个卵囊初始峰的1000L样品体积中获得40-60%的回收率。这项研究在简化样本浓度、提取和清理，以及提高隐孢子虫分析数据的重现性方面向前迈出了重要一步。包括11个参考文献、表格、图表。

The objective of this research was to develop a method of analysis for Cryptosporidium that would be simple to operate, low cost, and provide reproducible results that were not dependant upon the skill of the specific operator or the competence of the analysis laboratory. The research has focused on using a novel foam based filter Filta-Max(TM) that can be used to filter finished and raw waters. The filters are very compact, 5.5cm in diameter, 5cm deep, and fit a housing 10cm deep, thus making them ideal for use in the field. The filter eluent can be concentrated to approximately 20ml within 10 mins. without the need for centrifugation. The concentrates generated using the foam filters were then subjected to a novel immunomagnetic separtion (IMS) system with no requirement for centrifugation and resuspension. The novel IMS technology (Puri-Max[TM]) relies on the repeated circulation of the sample (plus paramagnetic beads) through a small capture phase, located in a magnetic field. The oocysts can be captured on the phase via antibody coated paramagnetic beads and the sample excess washed away. The oocysts are then simply recovered by removing them from the paramagnetic phase. Data is presented showing that with the combined Filta-Max/Puri-Max recoveries of between 40-60% can be obtained from sample volumes of 1,000L containing 100 oocyst initial spikes. This research represents a significant step forward in terms of simplifying sample concentration, extraction, and clean-up, and increasing the reproducibility of Cryptosporidium analysis data. Includes 11 references, tables, figures.