The scope of this study was to investigate how natural organic matter (NOM) from drinking water and soluble microbial products (SMP) generated during wastewater treatment engrave their character on TOC in water reuse systems. Higher source water TOC clearly correlated with higher reclaimed water TOC. Reclaimed water organic matter was lower in molecular weight and was only slightly more aromatic and less aliphatic as compared to drinking water from surface water sources. Although, residual organic carbon concentrations in reclaimed water are increased by soluble microbial products (SMPs) derived in the wastewater treatment process, the character of organic matter did not change according to XAD-8 and XAD-4 fractionation results. The similarity of drinking water and reclaimed water 13C-NMR spectra indicated that structure and composition of the isolates are dominated by NOM. Since TOC in reclaimed water is dominated by NOM which might vary depending on local source water situations, regulations should consider local drinking water concentrations.