流式细胞仪衍生的荧光测量用于评估四种抗体的批间变异性、交叉反应性和亲和力。试剂盒3和试剂盒4抗体批次内的变异性很大，而试剂盒1和试剂盒2始终对寄生虫进行染色。试剂盒3和4抗体与非微小隐孢子虫交叉反应，试剂盒1和4抗体与小鼠贾第虫交叉反应。试剂盒l（仅初级）、2和3抗隐孢子虫抗体形成低亲和力复合物，与试剂盒l（次级）、4和所有贾第虫抗体不同。此外，作者量化了抗体在方法处理后结合应激和非应激寄生虫的能力。研究发现，抗体特征和性能存在显著差异。包括27个参考文献、表格和图表。

Flow cytometer-derived fluorescence measurements were used to evaluate lot-to-lot variability, cross-reactivity, and avidity of four antibodies. Variability within kit 3 and 4 antibody lots was great, whereas kits 1 and 2 stained parasites consistently. Kit 3 and 4 anitbodies cross-reacted with non-parvum Cryptosporidium, kit l and 4 antibodies cross-reacted with Giardia muris. Kit l (primary only), 2, and 3 anti-cryptosporidium antibodies formed low-avidity complexes, unlike those in kits l (secondary) and 4 and all Giardia antibodies. Additionally, the authors quantified the ability of antibodies to bind stressed and unstressed parasites following methodological processing. The studies identified significant differences in antibody characteristics and performance. Includes 27 references, tables, figures.