1.1 本试验方法包括一种免疫学方法，使用对Guayule天然橡胶胶乳蛋白特异性的兔抗血清测定Guayule自然橡胶（GNR）及其产品中的蛋白质含量。该免疫测定程序使用抑制格式定量测量溶液中GNR蛋白的水平。样品可以包括但不限于手套或其他GNR产品提取物，以及为了测量蛋白质水平而收集的液体GNR样品。 1.2 就本试验方法而言，蛋白质的范围将以微克至毫克的量进行测量。 1.3 该测试方法可用于确定测试项目上是否存在GNR蛋白污染。 1.4 本标准并不旨在解决与其使用相关的所有安全问题（如有）。本标准的使用者有责任在使用前制定适当的安全、健康和环境实践，并确定监管限制的适用性。 1.5 本国际标准是根据世界贸易组织技术性贸易壁垒委员会发布的《关于制定国际标准、指南和建议的原则的决定》中确立的国际公认的标准化原则制定的。 ====意义和用途====== 5.1 NRL源自 海维雅 含有200多种蛋白质，其中许多是能够引发1型过敏反应的过敏原。Guayule天然橡胶源自 银Parthenium 这种植物所含的蛋白质比橡胶树NRL少得多，而且含量也低得多。虽然目前还没有关于GNR蛋白过敏反应的报道，但它们已被证明能在暴露的个体中诱导抗体的产生。 4. 测试GNR存在的免疫学测定具有提供其他ASTM标准如测试方法的特异性和敏感性的优点 D5712 和 D6499 不提供。 5.2 本试验方法描述了使用兔抗GNR血清定量Guayule天然橡胶蛋白的免疫学方法。用GNR蛋白免疫的兔子对存在的大多数蛋白质产生反应，并且它们的血清具有检测GNR中大多数（如果不是全部的话）蛋白质的能力。此测试方法可用于确定测试项目是否包含源自天然来源的材料。

1.1 This test method covers an immunological method to determine the amount of protein in Guayule Natural Rubber (GNR) and its products using rabbit antisera specific for Guayule Natural Rubber latex proteins. This immunoassay procedure quantitatively measures the level of GNR proteins in solution using an inhibition format. The samples may include but are not restricted to, gloves or other GNR product extracts, and liquid GNR samples which have been collected in order to measure the protein levels. 1.2 For the purpose of this test method, the range of protein will be measured in terms of microgram to milligram quantities. 1.3 This test method can be used to determine the presence of GNR protein contamination on test items. 1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. 1.5 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee. ====== Significance And Use ====== 5.1 NRL derived from Hevea contains over 200 proteins a number of which are allergens capable of eliciting Type 1 anaphylactic reactions. Guayule Natural Rubber derived from the Parthenium argentatum plant contains many fewer proteins and at much lower levels than Hevea NRL. While there have been no reports of allergic reactions to GNR proteins at present, they have been shown to induce antibody production in exposed individuals. 4 An immunological assay that tests for the presence of GNR has the advantage of providing the specificity and sensitivity that other ASTM standards such as Test Methods D5712 and D6499 do not offer. 5.2 This test method describes an immunological method for the quantitation of Guayule natural rubber proteins using rabbit anti-GNR serum. Rabbits immunized with GNR proteins react to the majority of the proteins present, and their sera have the capability to detect most if not all of the proteins in GNR. This test method may be used to determine if a test item contains material derived from a natural source.