用巨大芽孢杆菌孢子（B.megaterium，as）进行灭活实验 孢子）和枯草芽孢杆菌孢子（枯草芽孢杆菌，作为孢子）来研究灭活的差异 密切相关微生物之间的行为。结果表明，灭活行为 巨大双歧杆菌与枯草双歧杆菌的差异很大。巨大双歧杆菌没有表现出滞后期 如枯草杆菌所观察到的，巨大芽孢杆菌的失活动力学更快。在里面 此外，巨大双歧杆菌的失活似乎不依赖于pH值，而一些以前 已发表的研究表明枯草杆菌具有pH依赖性。这些细胞的失活反应 苏黎世湖水中的微生物与无需氧化剂的磷酸盐中的微生物不同 缓冲器两种微生物的物理反应都是在流动细胞中用原子吸收光谱法观察到的 力显微镜（AFM），可以在臭氧失活时恢复图像。 利用AFM可以清楚地观察到孢子被的氧化损伤。氧化损伤的开始 枯草芽孢杆菌孢子外壳的损伤明显晚于对细菌的氧化损伤 B.在类似臭氧暴露条件下的巨大细菌。包括16个参考文献、图表。

Inactivation experiments were conducted with Bacillus megaterium spores (B. megaterium, as spores) and Bacillus subtilis spores (B. subtilis, as spores) to investigate the differences in inactivation behavior among very closely related microorganisms. It was observed that the inactivation behavior of B. megaterium was very different than that of B. subtilis. B. megaterium did not exhibit a lag phase as observed with B. subtilis and the kinetics of inactivation were faster for B. megaterium. In addition, inactivation of B. megaterium does not appear to be pH dependent while some previously published research indicates a pH dependence of B. subtilis. The inactivation response of these microoganisms was different in Lake Zurich water than it was in oxidant demand free phosphate buffer. Physical responses of both microorganisms were observed in a flow through cell using Atomic Force Microscopy (AFM), which allowed images to be recovered while ozone inactivation occurred. Oxidative damage to the spore coat could be clearly observed using AFM. The onset of oxidative damage to the coat of B. subtilis spores occurred markedly later than the onset of oxidative damage to B. megaterium under similar ozone exposure conditions. Includes 16 references, figures.