1.1 本试验方法涵盖了在操作条件下评估荧光测量系统的荧光强度响应线性极限的程序。特别注意狭缝宽度、过滤器和样品容器。该测试方法可用于测试各种仪器和采样条件下的整体线性。所得结果仅适用于狭缝宽度和过滤器的测试组合，以及样品反应杯的尺寸、类型和照明度，所有这些都必须在报告中说明。非线性的来源可能是测量电子设备、激发或发射辐射的过度吸收，或两者兼有，以及样品处理技术，尤其是在低浓度下。 1.2 该测试方法已应用于使用连续和低能激发源（例如，450的激发源）的荧光测量系统 W电气输入或更少）。无法保证极强的照明不会导致本试验方法中建议的化合物发生光分解。 2. 因此，建议不要对高强度光源不加区分地使用本试验方法。它不是确定其他材料响应线性的测试方法。如果该试验方法扩展到使用其他化学物质，则可以应用中的原则，但必须建立新的材料参数，如线性浓度范围。 用户应注意这些其他物质可能会分解或吸附到容器上。 1.3 本试验方法已应用于使用单个探测器（即光电倍增管或单个光电二极管）的荧光测量系统。目前还没有证明这种方法对于使用CCD或二极管阵列探测器的光阵列仪器是否有效。 1.4 本试验方法适用于10 毫米波长范围在190毫米以内的反应杯格式和仪器 纳米至900纳米。本试验方法未确定使用其他样品格式。 1.5 以国际单位制表示的数值应视为标准值。 本标准不包括其他计量单位。 1.6 本标准并非旨在解决与其使用相关的所有安全问题（如有）。本标准的用户有责任在使用前制定适当的安全、健康和环境实践，并确定监管限制的适用性。 1.7 本国际标准是根据世界贸易组织技术性贸易壁垒（TBT）委员会发布的《关于制定国际标准、指南和建议的原则的决定》中确立的国际公认标准化原则制定的。 ====意义和用途====== 3.1 荧光物质在溶液中的浓度范围（荧光随浓度线性变化）是定量分析最有用的范围。该范围受分析溶液的特性和测量系统的特性的影响。本试验方法提供了一种测试荧光测量系统性能的方法，以及确定系统适合进行给定定量分析的浓度范围的方法。 3.2 本试验方法不用于比较不同荧光测量仪器的性能。

1.1 This test method covers a procedure for evaluating the limits of the linearity of response with fluorescence intensity of fluorescence-measuring systems under operating conditions. Particular attention is given to slit widths, filters, and sample containers. This test method can be used to test the overall linearity under a wide variety of instrumental and sampling conditions. The results obtained apply only to the tested combination of slit width and filters, and the size, type and illumination of the sample cuvette, all of which must be stated in the report. The sources of nonlinearity may be the measuring electronics, excessive absorption of either the exciting or emitted radiation, or both, and the sample handling technique, particularly at low concentrations. 1.2 This test method has been applied to fluorescence-measuring systems utilizing continuous and low-energy excitation sources (for example, an excitation source of 450 W electrical input or less). There is no assurance that extremely intense illumination will not cause photodecomposition of the compounds suggested in this test method. 2 For this reason it is recommended that this test method not be indiscriminately employed with high-intensity light sources. It is not a test method to determine the linearity of response of other materials. If this test method is extended to employ other chemical substances, the principles within can be applied, but new material parameters, such as the concentration range of linearity, must be established. The user should be aware of the possibility that these other substances may undergo decomposition, or adsorption onto containers. 1.3 This test method has been applied to fluorescence-measuring systems utilizing a single detector, that is, a photomultiplier tube or a single photodiode. It has not been demonstrated if this method is effective for photo-array instruments such as those using a CCD or a diode array detector. 1.4 This test method is applicable to 10 mm pathlength cuvette formats and instruments covering a wavelength range within 190 nm to 900 nm. The use of other sample formats has not been established with this test method. 1.5 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard. 1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. 1.7 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee. ====== Significance And Use ====== 3.1 The range of concentration of a fluorescing substance in solution over which the fluorescence varies linearly with the concentration is the range most useful for quantitative analysis. This range is affected by properties of the solution under analysis and by features of the measuring system. This test method provides a means of testing the performance of a fluorescence measuring system and of determining the concentration range over which the system is suitable for making a given quantitative analysis. 3.2 This test method is not meant for comparing the performance of different fluorescence measuring instruments.