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Standard Test Method for Determination of Carbohydrates in Biomass by High Performance Liquid Chromatography 用高效液相色谱法测定生物质中碳水化合物的标准试验方法
发布日期: 2024-05-01
1.1 该试验方法包括测定生物质样品中存在的碳水化合物,用105 °C干燥质量基准。 注1: 在计算原始生物质样品中多糖的实际质量百分比之前,必须针对水解水校正糖百分比。 1.2 适用于该程序的样品材料包括硬木材和软木材、草本材料(如柳枝稷和蚕桑)、农业残留物(如玉米秸秆、小麦秸秆和甘蔗渣)、废纸(如办公垃圾、纸板和新闻纸)、酸性或碱性- 预处理的生物质(洗涤时没有任何残留的酸或碱)和发酵残留物的固体部分。所有结果都是相对于105的质量报告的 °C烘箱干燥的样品固体。 1.3 本试验方法中待分析样品类型的选项如下: 1.3.1 制备的生物质样品: 1.3.1.1 空气干燥(%T 广告 )— 空气干燥样品总固体的质量百分比。 1.3.1.2 45 °C 干燥(%T 45 )--45的总固体的质量百分比 °C干燥样品。 1.3.1.3 冷冻干燥(%T fd )— 冷冻干燥样品总固体的质量百分比。 1.3.2 提取物游离样品(%T 提取 )— 在105测定的提取样品总固体的质量百分比 °C。 1.4 以国际单位制表示的数值应视为标准。 1.5 本标准并不旨在解决与其使用相关的所有安全问题(如有)。本标准的使用者有责任在使用前制定适当的安全、健康和环境实践,并确定监管限制的适用性。 中给出了具体的预防说明 11.5 和 11.10 . 1.6 本国际标准是根据世界贸易组织技术性贸易壁垒委员会发布的《关于制定国际标准、指南和建议的原则的决定》中确立的国际公认的标准化原则制定的。 ====意义和用途====== 4.1 糖含量的质量百分比与其他测定法结合使用,以确定生物质样品的总组成。
1.1 This test method covers the determination of carbohydrates present in a biomass sample, expressed as the percent, by mass, of each sugar on a 105 °C dried mass basis. Note 1: The percent sugar must be corrected for the water of hydrolysis before calculating the actual mass percent of the polysaccharide in the original biomass sample. 1.2 Sample materials suitable for this procedure include hard and soft woods, herbaceous materials (such as switchgrass and sericea), agricultural residues (such as corn stover, wheat straw, and bagasse), wastepaper (such as office waste, boxboard, and newsprint), acid or alkaline-pretreated biomass (washed free of any residual acid or alkali), and the solid fraction of fermentation residues. All results are reported relative to the mass of the 105 °C oven-dried solids of the sample. 1.3 The options for the types of samples to be analyzed in this test method are as follows: 1.3.1 Prepared Biomass Samples: 1.3.1.1 Air Dried (%T ad )— The percent, by mass, of total solids of the air-dried sample. 1.3.1.2 45 °C Dried (%T 45 )—The percent, by mass, of total solids of the 45 °C dried sample. 1.3.1.3 Freeze Dried (%T fd )— The percent, by mass, of total solids of the freeze dried sample. 1.3.2 Extractives-Free Sample (%T ext )— The percent, by mass, of total solids of the extracted sample determined at 105 °C. 1.4 The values stated in SI units are to be regarded as the standard. 1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. Specific precautionary statements are given in 11.5 and 11.10 . 1.6 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee. ====== Significance And Use ====== 4.1 The percentage, by mass, of sugar content is used in conjunction with other assays to determine the total composition of biomass samples.
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