Cosmetics — Microbiology — Enumeration of yeast and mould
化妆品 - 微生物学 - 酵母和模具的计数
发布日期:
2017-06-14
ISO 16212:2017通过在有氧培养后在选择性琼脂培养基上计数菌落,给出了化妆品中酵母和霉菌计数的一般指南。
为了确保消费者的产品质量和安全,建议进行适当的微生物风险分析,以确定ISO 16212:2017适用的化妆品类型。被认为具有低微生物风险的产品(见ISO 29621)包括低水活性或极端pH值的产品、水酒精产品等。
由于该应用领域的化妆品种类繁多,该方法可能不适用于某些产品的每个细节(例如某些水不溶性产品)。如果已证明其等效性,或已证明该方法适用,则可使用其他方法(如自动方法)代替此处提供的测试。
可以使用合适的鉴定试验来鉴定列举的酵母,例如参考文献中列出的标准中所述的试验。
如有必要,可通过其他适当的方法识别列举的霉菌。
ISO 16212:2017 gives general guidelines for enumeration of yeast and mould present in cosmetics by counting the colonies on selective agar medium after aerobic incubation.
In order to ensure product quality and safety for consumers, it is advisable to perform an appropriate microbiological risk analysis to determine the types of cosmetic products to which ISO 16212:2017 is applicable. Products considered to present a low microbiological risk (see ISO 29621) include those with low water activity or extreme pH values, hydro-alcoholic products, etc.
Because of the large variety of cosmetic products within this field of application, this method might not be suited to some products in every detail (e.g. certain water-immiscible products). Other methods (e.g. automated) can be substituted for the tests presented here provided that their equivalence has been demonstrated or the method has been otherwise shown to be suitable.
Yeast enumerated can be identified using suitable identification tests, for example, tests described in the standards listed in the Bibliography. Mould enumerated can be identified by other appropriate methods, if necessary.