传统化学消毒对微小隐孢子虫等病原体的灭活效果有限，强调了颗粒介质过滤等物理化学过程在有效水处理中的关键作用。越来越严格的法规，如预期的长期2强化地表水处理规则（LT2ESWTR），可能需要证明处理性能，或纳入额外的处理过程，以去除病原体和/或灭活。由于本地微小隐孢子虫浓度通常较低，因此通常利用种子中的微小隐孢子虫浓度进行研究。 微小卵囊或替代指标。然而，随着高种子浓度的使用，出现了一个问题，即根据基于极高进水卵囊浓度的研究，推断处理低进水卵囊浓度水的过滤器的微小隐球菌去除能力是否合适。在几个操作条件下，利用高和低种子微小隐孢子虫和聚苯乙烯微球浓度（约两个数量级）进行实验，研究了颗粒介质和硅藻土（DE）过滤去除微小隐孢子虫的效果。 在稳定运行期间，观察到颗粒培养基和脱滤对微小隐球菌的良好去除效果。在所研究的范围内，种子浓度似乎不会影响颗粒培养基或反过滤去除微小隐孢子虫。实验还表明，聚苯乙烯微球作为过滤去除微小隐球菌的定量替代物的适用性存在一些局限性。包括9个参考文献、图表。

The limited efficacy of traditional chemical disinfection for inactivating pathogens such as Cryptosporidium parvum emphasizes the critical role of physico-chemical processes such as granular media filtration for effective water treatment. Increasingly stringent regulations such as the anticipated Long-Term 2 Enhanced Surface Water Treatment Rule (LT2ESWTR) may require the demonstration of treatment performance or the incorporation of additional treatment processes for pathogen removal and/or inactivation. Since indigenous C. parvum concentrations are typically low, studies are often performed utilizing seeded concentrations of C. parvum oocysts or surrogate indicators. With the use of high seeded concentrations, however, comes the question of whether it is appropriate to extrapolate the C. parvum removal capacities of filters treating waters with low influent concentrations of oocysts from studies based on extremely high influent oocyst concentrations. C. parvum removals by granular media and diatomaceous earth (DE) filtration were investigated during experiments utilizing high and low seeded C. parvum and polystyrene microsphere concentrations (approximately two orders of magnitude) during several operating conditions. Excellent C. parvum removals by both granular media and DE filtration during stable operation were observed. Over the range investigated, seeded concentrations did not appear to impact C. parvum removal by either granular media or DE filtration. The experiments also suggested some limitations in the applicability of polystyrene microspheres as quantitative surrogates for C. parvum removal by filtration. Includes 9 references, figures.