ISO 16050:2003规定了一种反相高效液相色谱法，其具有免疫亲和柱清洗和柱后衍生，用于测定谷物，坚果和衍生产品中的黄曲霉毒素。黄曲霉毒素B1和黄曲霉毒素B1，B2，G1和G2之和的量化限值为每公斤8微克。 该方法已经验证了每公斤24,5微克的玉米，每公斤8,4微克的花生酱和每公斤总黄曲霉毒素16微克的生花生。也已经表明，该方法可用于油籽产品，干果和衍生产品

ISO 16050:2003 specifies a reverse-phase high-performance liquid chromatographic method, with immunoaffinity column clean-up and post-column derivatization, for the determination of aflatoxins in cereals, nuts and derived products. The limit of quantification for aflatoxin B1, and for the sum of aflatoxins B1, B2, G1 and G2, is 8 micrograms per kilogram. The method has been validated for maize containing 24,5 micrograms per kilogram, for peanut butter containing 8,4 micrograms per kilogram, and for raw peanuts containing 16 micrograms per kilogram of total aflatoxins. It has also been shown that this method can be used for oilseed products, dried fruits and derived products.