对17个州的150个地下水样本进行了研究，以解决地下水中病毒的发生、传染性和识别问题。所有样本均通过常规细胞培养进行分析，以确定地下水中是否存在传染性病毒。使用BGM细胞系，22个样本表现出对感染性病毒的细胞病变效应。然后通过RT-PCR检测细胞培养收获物，以进行病毒检测和鉴定。使用肠道病毒、轮状病毒、呼肠孤病毒、甲型肝炎病毒和诺沃克病毒的单独引物组对样本进行检测。包括7个参考文献和表格。

A study of 150 groundwater samples from sites in 17 states was undertaken to address the occurrence, infectivity, and identification of viruses in groundwater. All samples were analyzed by conventional cell culture to determine the occurrence of infectious viruses in groundwater sites. Twenty-two samples exhibited cytopathic effect for infectious viruses using BGM cell line. The cell culture harvests were then assayed by RT-PCR for viral detection and identification. The samples were examined using separate primer sets for enterovirus, rotavirus, reovirus, hepatitis A virus, and Norwalk virus. Includes 7 references, tables.