本文件规定了与食品链微生物学定量结果相关的测量不确定度（MU）的估算和表示的要求和指南。适用于:-供人食用或喂饲动物的产品；-食品生产和食品加工领域的环境样品；-初级生产阶段的样品。定量分析通常通过使用菌落计数技术对微生物进行计数来进行。本文件通常也适用于其他定量分析，包括:-最可能数（MPN）技术；-阻抗法、三磷酸腺苷（ATP）和流式细胞术等仪器方法；-分子方法，如基于定量聚合酶链反应（qPCR）的方法。本文件估计的不确定性不包括系统影响（偏差）。

This document specifies requirements and gives guidance for the estimation and expression of measurement uncertainty (MU) associated with quantitative results in microbiology of the food chain. It is applicable to the quantitative analysis of: — products intended for human consumption or the feeding of animals; — environmental samples in the area of food production and food handling; — samples at the stage of primary production. The quantitative analysis is typically carried out by enumeration of microorganisms using a colony-count technique. This document is also generally applicable to other quantitative analyses, including: — most probable number (MPN) techniques; — instrumental methods, such as impediometry, adenosine triphosphate (ATP) and flow cytometry; — molecular methods, such as methods based on quantitative polymerase chain reaction (qPCR). The uncertainty estimated by this document does not include systematic effects (bias).