新鲜的贾第虫囊肿和隐孢子虫卵囊加入10升试剂或地表水中，然后使用Envirochek（TM）过滤器过滤，使用Dynabeads进行IMS（免疫磁分离）以测定平均回收率。还评估了两种不同的荧光标记抗体:微小隐球菌和小鼠隐球菌。印度环境管理部根据EPA方法1623说明收集了10升河流样本，并在72小时内进行了分析。使用M-ColiBlue24检测大肠杆菌，使用改良mCP琼脂分析产气荚膜梭菌。在本研究中，指示菌的结果和隐孢子虫和贾第鞭毛虫的浓度无关。 包括8个参考文献、表格和图表。

Fresh Giardia cysts and Cryptosporidium oocysts were spiked into 10 liters ofreagent or surface water, then followed by filtration using Envirochek (TM)filters, and IMS (immunomagnetic separation) using Dynabeads for determination ofmean recoveries. Two different fluorescent labeled antibodies were also evaluated,C. parvum and C. muris. Ten liters of river samples were collected by the IndianaDepartment of Environmental Management according to the EPA Method 1623instructions and analyzed within 72 hours. Escherichia coli was detected usingm-ColiBlue24 and C. perfringens were analyzed using modified mCP agar. Results ofindicator bacteria are presented and do not correlate with Cryptosporidium andGiardia concentrations in this study. Includes 8 references, tables, figure.