本文件规定了脂肪酸甲酯（FAME）中游离甘油和残余单、双和甘油三酯含量的测定方法。然后根据所得结果计算总甘油含量。 在所述条件下，游离甘油的定量限为0001%（m/m），所有甘油酯（一元、二元和三元）的定量限为0.10%（m/m）。本法适用于菜籽、向日葵、大豆、棕榈油、动物油脂及其混合物制备脂肪酸甲酯。由于不同甘油酯峰重叠，不适用于由椰子油和棕榈仁油衍生物生产或含有椰子油和棕榈仁油衍生物的脂肪酸。 注1:在本文件中，术语“%（m/m）”用于表示质量分数。 注2:在常见的EN 14105气相色谱条件下，角鲨烯可与α-甘油单硬脂酸盐共存。如果怀疑存在角鲨烯，可使用EN 17057区分角鲨烯和单硬脂酸甘油酯。 警告-使用本文件可能涉及危险材料、操作和设备。本文件并不旨在解决与其使用相关的所有安全问题。本文件的使用者有责任在应用本标准之前采取适当措施确保人员的安全和健康，并满足相关的法律法规要求。

This document specifies a method to determine the free glycerol and residual mono-, di- and triglyceride contents in fatty acid methyl esters (FAME). The total glycerol content is then calculated from the obtained results. Under the conditions described, the quantification limits are 0,001 % (m/m) for free glycerol, 0,10 % (m/m) for all glycerides (mono-, di- and tri-). This method is suitable for FAME prepared from rapeseed, sunflower, soybean, palm, animal oils and fats and mixture of them. It is not suitable for FAME produced from or containing coconut and palm kernel oils derivatives because of overlapping of different glyceride peaks. NOTE 1 For the purposes of this document, the term "% (m/m)" is used to represent the mass fraction. NOTE 2 Under the common EN 14105 GC conditions squalene can coelute with alpha glycerol monostearate. If the presence of squalene is suspected, EN 17057 can be used to discriminate between squalene and glycerol monostearate. WARNING - The use of this document can involve hazardous materials, operations and equipment. This document does not purport to address all of the safety problems associated with its use. It is the responsibility of users of this document to take appropriate measures to ensure the safety and health of personnel prior to application of the standard, and fulfil statutory and regulatory requirements for this purpose.