本研究的目的是比较微小隐孢子虫卵囊在CD-1小鼠、HCT-8细胞、MDCK细胞和Caco-2细胞中的感染性，以确定不同模型对感染的敏感性是否不同，产生不同的剂量反应，或测量卵囊失活的能力是否不同。总的来说，细胞培养至少与CD-1小鼠模型一样敏感，可用于测量新鲜卵囊的传染性。然而，所有模型中都存在相当大的差异。在某些情况下，细胞培养得出的剂量-反应曲线的形状与小鼠的曲线无法区分。对于所有基因型2分离株（n=5），Caco-2细胞系对感染的耐受性最低。5个分离物的ID50值范围为21- CD-1小鼠215例，HCT-8细胞19-87例，MDCK细胞2-85例，Caco-2细胞43-327例。基因型1分离物（在小鼠中非传染性）在HCT-8细胞中有631个卵囊的ID50，尽管这无法与基因型2分离物的数据直接比较，因为基因型1卵囊相当古老，并且在非理想条件下储存。这是首次使用精确计数的卵囊剂量，将细胞培养与微小隐球菌动物模型进行严格比较，五个分离株的结果表明，细胞培养中的传染性可能是卵囊在动物中引起感染的能力的良好指标。这项研究表明，体外细胞培养相当于测量C。 微小卵囊。包括19个参考文献、表格、图表。

The objective of this investigation were to compare infectivity of Cryptosporidium parvum oocysts in CD-1 mice, HCT-8 cells, MDCK cells, and Caco-2 cells, to determine whether the different models vary in their sensitivity to infection, generate different dose responses, or vary in their ability to measure oocyst inactivation. In general, cell culture was at least as sensitive as the CD-1 mouse model for measuring the infectivity of fresh oocysts. However, considerable variation existed in all models. In some instances the shape of the dose response curve derived from cell culture was indistinguishable from the curve for mice. For all genotype 2 isolates (n = 5), the Caco-2 cell line was least receptive to infection. The ID50 values for the 5 isolates ranged from 21 - 215 in CD-1 mice, 19 - 87 in HCT-8 cells, 2 - 85 in MDCK cells, and 43 - 327 in Caco-2 cells. A genotype 1 isolate (non-infectious in mice) had an ID50 of 631 oocysts in HCT-8 cells, although this could not be directly compared with the data for genotype 2 isolates since the genotype 1 oocysts were considerably older and stored under non-ideal conditions. This is the first rigorous comparison of cell culture with an animal model for C. parvum, using precisely enumerated oocyst doses, and the results with five isolates demonstrated that infectivity in cell culture may be a good indicator of the ability of oocysts to cause infection in animals. This study demonstrated that in-vitro cell culture is equivalent to a standard mouse model for measuring infectivity of C. parvum oocysts. Includes 19 references, table, figures.