1.1本规程提供了分光光度法，用于检测物体颜色样本中是否存在荧光。 笔记 1-由于材料制造商通常有意添加荧光剂（着色剂、增白剂等），因此通常可以从材料制造商处获得样品中是否存在荧光特性的信息。 1.2本规程要求使用分光光度计，该分光光度计在340至700 nm的波长范围内照射样品，并允许根据需要改变样品上照明的光谱分布。 1.3在上述限制范围内，本惯例在范围上是通用的，而不是特定于仪器或材料。 1.4 本标准并非旨在解决与其使用相关的所有安全问题（如有）。本标准的用户有责任在使用前制定适当的安全和健康实践，并确定监管限制的适用性。 ====意义和用途====== 包括实践在内的多个标准 E991 , E1164 和试验方法 E1331 , E1348 和 E1349 ，要求样品显示荧光的存在或不存在，以便正确应用。本规程提供了用于识别材料中荧光存在的分光光度程序。 本规程适用于所有物体颜色样本，无论是不透明、半透明还是透明，均符合2.1中所列适当标准中对样本的要求。半透明试样应通过反射率进行测量，在测量过程中，将标准非荧光背衬材料（通常但不一定是黑色）放置在试样后面。 本规程要求使用分光光度计，其中用户可以通过以下几种方式之一改变样品上照明的光谱分布。照明的修改可以通过在照明源和样本之间插入滤光片，而不干扰样本辐射的检测，或者通过交换仪器的照明和检测系统，或者通过扫描照明能量和检测输出，如两种方法所示- 单色仪法。 通过比较光谱曲线、色差或单参数差异（例如 Δ 测量值之间的Y。 笔记 2-在E1247-92和更早版本中，荧光测试是两组光谱透射率或辐射因子（反射因子）在最大差异波长处相差满标度的1%。 本实践中可以使用双向或半球仪器几何形状。仪器必须能够在样品上提供宽带（白光）辐照或单色辐照和单色检测。 本规程描述了仅检测荧光存在的方法。它没有解决荧光对任何给定应用的样品色度特性的贡献是否显著或不显著的问题。用户必须确定荧光对颜色测量影响的实际意义。

1.1 This practice provides spectrophotometric methods for detecting the presence of fluorescence in object-color specimens. Note 1—Since the addition of fluorescing agents (colorants, whitening agents, etc.) is often intentional by the manufacturer of a material, information on the presence or absence of fluorescent properties in a specimen may often be obtained from the maker of the material. 1.2 This practice requires the use of a spectrophotometer that both irradiates the specimen over the wavelength range from 340 to 700 nm and allows the spectral distribution of illumination on the specimen to be altered as desired. 1.3 Within the above limitations, this practice is general in scope rather than specific as to instrument or material. 1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. ====== Significance And Use ====== Several standards, including Practices E991 , E1164 , and Test Methods E1331 , E1348 and E1349 , require either the presence or absence of fluorescence exhibited by the specimen for correct application. This practice provides spectrophotometric procedures for identifying the presence of fluorescence in materials. This practice is applicable to all object-color specimens, whether opaque, translucent, or transparent, meeting the requirements for specimens in the appropriate standards listed in 2.1. Translucent specimens should be measured by reflectance, with a standard non-fluorescent backing material, usually but not necessarily black, placed behind the specimen during measurement. This practice requires the use of a spectrophotometer in which the spectral distribution of the illumination on the specimen can be altered by the user in one of several ways. The modification of the illumination can either be by the insertion of optical filters between the illuminating source and the specimen, without interfering with the detection of the radiation from the specimen, or by interchange of the illuminating and detecting systems of the instrument or by scanning of both the illuminating energy and detection output as in the two-monochromator method. The confirmation of the presence of fluorescence is made by the comparison of spectral curves, color difference, or single parameter difference such as Δ Y between the measurements. Note 2—In editions of E1247 - 92 and earlier, the test of fluorescence was the two sets of spectral transmittances or radiance factor (reflectance factors) differ by 1 % of full scale at the wavelength of greatest difference. Either bidirectional or hemispherical instrument geometry may be used in this practice. The instrument must be capable of providing either broadband (white light) irradiation on the specimen or monochromatic irradiation and monochromatic detection. This practice describes methods to detect the presence of fluorescence only. It does not address the issue of whether the fluorescence makes a significant or insignificant contribution to the colorimetric properties of the specimen for any given application. The user must determine the practical significance of the effect of fluorescence on the color measurement.