The objective of this study was to investigate the effect of water quality parameters and ozone dosage on formaldehyde formation during interaction of ozone with natural organic matter. Humic and fulvic acids are selected as natural organic matter models, mainly because they are the major constituents of natural organic matter, and they may be the primary sources of precursor material for aldehydes. Ozonation was tested on synthetic solutions of natural humic and fulvic acids under various conditions. MQ-Untra Low total organic carbon (TOC) water was used to prepare the test solutions in order to achieve a 2-3 ug/L formaldehyde concentration in the background. MQ-Ultra Low TOC system exposes the deionized water to UV light at both 185 and 254 nm wavelengths, thus oxidizing organic compounds and killing bacteria. Purified water then passes through an Ultrafiltration (UF) module, which acts as a barrier to colloids, particles and organic molecules with a molecular weight greater than 5000 Dalton. This research attempts to provide specific information on the effect of process parameters and water quality characteristics on oxidation of humic substances and generation of formaldehyde.