几种实时定量PCR方法，包括直接PCR和RT-PCR 方法，以及基于PCR和RT-PCR结合组织细胞培养的方法 （ICC-PCR和ICC-RT-PCR）用于评估紫外线（UV）灭活腺病毒41 （公元41年）。在HEK293细胞上生长的Ad 41（TAK菌株）暴露于40， 80、160和320 mJ/cm²使用准直光束装置。当与 基于传统细胞病变效应（CPE）的细胞培养传染性分析，直接实时 定量PCR和RT-PCR检测（即无细胞培养）被发现为 无法通过紫外线测量Ad41的失活水平。失去生命的病毒 感染性仍然通过直接分子方法检测。Ad41的紫外线灭活 另一方面，ICC-RT-PCR检测结果与对照组无统计学差异 通过CPE分析测定失活。ICCPCR检测Ad41的紫外线灭活 检测结果略低于CPE和ICC-RT-PCR检测的失活率。 因此，一种基于病毒定量的细胞培养分析方法使用实时定量技术 RT-PCR被发现是基于CPE的细胞培养的潜在有吸引力的替代品 用于评估紫外线灭活病毒的传染性试验。包括11个参考文献、表格、图表。

Several real-time quantitative PCR methods, including direct PCR and RT-PCR methods, and methods based on PCR and RT-PCR integrated with tissue cell culture (ICC-PCR and ICC-RT-PCR) were used to evaluate ultraviolet (UV) inactivation of adenovirus 41 (Ad 41). Ad 41 (TAK strain) grown on HEK293 cells were exposed to UV doses of 40, 80, 160 and 320 mJ/cm² using a collimated beam apparatus. When compared to the traditional cytopathic effects (CPE) based cell culture infectivity assay, direct real-time quantitative PCR and RT-PCR assays (i.e. without cell culture) were found to be unreliable for measuring the level inactivation of Ad41 by UV. Viruses which lost their infectivity were still detected by the direct molecular methods. UV inactivation of Ad41 measured by ICC-RT-PCR, on the other hand, was not statistically different than inactivation measured by the CPE assay. UV inactivation of Ad41 measured by the ICCPCR assay was slightly lower than inactivation measured by CPE and ICC-RT-PCR. Therefore, a cell culture assay based on viral quantification using real-time quantitative RT-PCR was found to be a potentially attractive substitute for the CPE-based cell culture infectivity assay for assessing virus inactivation by UV. Includes 11 references, table, figures.