本文件规定了一种从土壤样品中直接提取DNA的方法，以通过各种分子生物学技术（包括实时定量PCR（qPCR））分析微生物群落的丰度和组成。这种方法主要用于农业和森林土壤。这种方法可能不适用于富含有机质的土壤（例如泥炭土）或被有机污染物或重金属严重污染的土壤。购买本文件时可获得的所有当前修订均包含在购买本文件中。

The present document specifies a method for direct extraction of DNA from soil samples to analyse the abundance and composition of microbial communities by various techniques of molecular biology including real-time quantitative PCR (qPCR). This method is mainly dedicated to agricultural and forest soils. This method can possibly not be suitable for soils rich in organic matter (e.g. peat soils) or soils heavily polluted with organic pollutants or heavy metals.All current amendments available at time of purchase are included with the purchase of this document.