ISO 12966-4:2015规定了通过毛细管气相色谱法（GLC）测定脂肪、油和脂肪酸酯交换或酯化产生的脂肪酸甲酯（FAME）的方法。使用ISO 12966的这一部分可以分离C8到C24的脂肪酸甲酯，包括饱和脂肪酸甲酯、顺式和反式单不饱和脂肪酸甲酯以及顺式和反式多不饱和脂肪酸甲酯。 该方法适用于从动物和植物源中提取的粗、精制、部分氢化或完全氢化的脂肪、油和脂肪酸。 该方法不适用于乳制品、反刍动物脂肪和油，或添加共轭亚油酸（CLA）的产品的分析。 牛奶和奶制品（或来自牛奶和奶制品的脂肪）不在ISO 12966本部分的范围内。 ISO 12966-4:2015不适用于二、三、聚合和氧化脂肪酸以及脂肪和油。

ISO 12966-4:2015 specifies a method for the determination of fatty acid methyl esters (FAMEs) derived by transesterification or esterification from fats, oils, and fatty acids by capillary gas chromatography (GLC). Fatty acid methyl esters from C8 to C24 can be separated using this part of ISO 12966 including saturated fatty acid methyl esters, cis- and trans-monounsaturated fatty acid methyl esters, and cis- and trans-polyunsaturated fatty acid methyl esters. The method is applicable to crude, refined, partially hydrogenated, or fully hydrogenated fats, oils, and fatty acids derived from animal and vegetable sources. This method is not suitable for the analysis of dairy, ruminant fats and oils, or products supplemented with conjugated linoleic acid (CLA). Milk and milk products (or fat coming from milk and milk products) are excluded from the scope of this part of ISO 12966. ISO 12966-4:2015 is not applicable to di-, tri-, polymerized and oxidized fatty acids, and fats and oils.