由于MS2大肠杆菌噬菌体是北美紫外线（UV）反应器验证最常用的试验微生物，在实验室规模上开发MS2的紫外线剂量响应数据是验证测试的一个组成部分。在这项研究中，对17个过滤水的水质和样品深度对MS2大肠杆菌噬菌体灭活的影响进行了评估。该研究还比较了低压（LP）和中压（MP）灯的灭活性能。研究结果表明，在通常用于水处理的紫外线剂量下，浊度、颗粒数和大小不会显著影响过滤水中种子MS2的LP和MP消毒效率。 MP灯发出的紫外光在灭活MS2方面似乎比LP UV更有效，但需要进行额外的研究，以确定MP UV是否确实更有效，或者MP剂量计算方法是否需要进一步改进。作者建议，作为生物剂量学反应堆验证试验方案的一部分进行的台架试验，将最大样品深度设定为1厘米，使用LP单色紫外线灯，并遵循公认的辐照度校正方法。紫外线照射被认为是一种行之有效的消毒水源的方法。本研究为水供应商提供了评估MS2大肠杆菌噬菌体在试验台上使用的工具- 紫外线反应器测试的规模测试组件。此外，研究结果应有助于提高在过滤水中使用MS大肠杆菌噬菌体进行紫外线反应器验证的实用性信心。

With MS2 coliphage the most commonly used test microbe for ultraviolet (UV) reactor validation in North America, the development of UV dose-response data for MS2 on the bench scale is an integral part of validation testing. In this research, the effects of water quality and sample depth on inactivation of MS2 coliphage were evaluated for 17 filtered waters. The study also compared the inactivation performance of low-pressure (LP) and medium-pressure (MP) lamp types. Study findings indicated that at the UV doses customarily used in water treatment, turbidity and particle count and size do not significantly affect LP and MP disinfection efficiency of seeded MS2 in filtered water. UV light from MP lamps appeared more effective than LP UV for inactivating MS2, but additional research is necessary to determine if MP UV is actually more effective or if the MP dose calculation method needs further refinement. The authors recommend that bench testing performed as part of a biodosimetry reactor validation test protocol set a maximum sample depth of 1 cm, use LP monochromatic UV lamps, and follow accepted irradiance correction methods. UV light irradiation is recognized as a proven method for disinfecting water supplies. This study offers water providers tools to evaluate the use of MS2 coliphage for the bench-scale testing component of UV reactor testing. In addition, research results should help increase utility confidence in the use of MS coliphage for UV reactor validation in filtered waters.