本文采用高效液相色谱-串联质谱法（MS\/MS），对食品中黄曲霉毒素B1、脱氧雪腐镰刀菌烯醇、伏马菌素B1和B2、赭曲霉毒素A、HT-2和T-2毒素和玉米赤霉烯酮的筛选方法进行了研究。筛选方法的目的是测试食品是否符合监管限值，或确定是否超过某一预先定义的水平（筛选目标浓度，STC）。筛查结果不是“阴性”就是“可疑”阴性（筛检阴性）意味着未检测到或可能存在的靶向真菌毒素，但低于STC。可疑（“屏幕阳性”）意味着超过所建立的截止水平，并且样本可以包含比STC高的一个或多个真菌毒素。为了充分识别和准确定量，需要第二种验证性定量分析方法，该方法不在本文件的范围内。该方法适用于各种类型的食品，并已对四种商品组的代表性基质进行了验证:-淀粉和\/或蛋白质含量高，水分和脂肪含量低:小麦、谷类混合物、小麦粉和玉米片；-含油量高:花生；-高糖低水分:无花果；-含水量高:葡萄汁。在验证过程中，确定了以下筛选目标浓度的截止值:黄曲霉毒素B1:2μg\/kg至5μg\/kg；-脱氧雪腐镰刀菌烯醇:250微克\/千克至865微克\/千克；-伏马菌素B1:200微克\/千克至790微克\/千克；-伏马菌素B2:110微克\/千克至230微克\/千克；赭曲霉毒素A:4μg\/kg至9μg\/kg；T-2毒素:25μg\/kg；-HT-2毒素:25μg\/kg至50μg\/kg；-玉米赤霉烯酮:30微克\/千克至100微克\/千克。

This document describes a screening method for the determination of aflatoxin B1, deoxynivalenol, fumonisin B1 and B2, ochratoxin A, HT-2 and T-2 toxins, and zearalenone in foodstuffs by high performance liquid chromatography (HPLC) coupled with tandem mass spectrometry (MS/MS). The aim of the screening method is to test compliance of foodstuff with regulatory limits or to determine whether a certain pre-defined level (the screening target concentration, STC) is exceeded or not. The result of the screening is either "negative" or "suspect". "Negative" (screen negative) means that the targeted mycotoxins are not detected or potentially present but below the STC. "Suspect" (screen positive) means that the established cut-off level is exceeded and the sample can contain one or more mycotoxins at a level higher than the STC. For full identification and accurate quantification a second confirmatory quantitative analysis method is required which is outside the scope of this document. The method is suitable for various types of foodstuff and has been validated for representative matrices from four commodity groups: - high starch and/or protein content and low water and fat content: wheat, cereal mixture, wheat flour and cornflakes; - high oil content: peanuts; - high sugar low water content: figs; - high water content: grape juice. During validation, cut-off levels were established for the following screening target concentrations: - aflatoxin B1: 2 µg/kg to 5 µg/kg; - deoxynivalenol: 250 µg/kg to 865 µg/kg; - fumonisin B1: 200 µg/kg to 790 µg/kg; - fumonisin B2: 110 µg/kg to 230 µg/kg; - ochratoxin A: 4 µg/kg to 9 µg/kg; - T-2 toxin: 25 µg/kg; - HT-2 toxin: 25 µg/kg to 50 µg/kg; - zearalenone: 30 µg/kg to 100 µg/kg.