本文件规定了通过分子方法检测核酸序列（DNA）的最低性能要求，如聚合酶链反应（PCR），包括不同的PCR后检测方法、实时PCR、单探针和/或多探针检测技术以及这些方法的组合。 本文件适用于食品中较高和较低分类群动物物种DNA的检测、鉴定和定量，以及适用方法的验证。 适用于哺乳动物、鸟类、爬行动物、两栖动物、鱼类、软体动物、甲壳类动物和昆虫。附录A中列出了每种方法的典型示例。

This document specifies minimum requirements of performance characteristics for the detection of nucleic acid sequences (DNA) by molecular methods, such as the polymerase chain reaction (PCR), including different post-PCR detection methods, real-time PCR, single and/or multiple probe-based detection techniques as well as the combination of such methods. The document is applicable to the detection, identification and quantification of DNA from animal species of higher and lower taxonomic groups in foodstuffs, and the validation of applicable methods. It is applicable to mammals, birds, reptiles, amphibians, fishes, molluscs, crustaceans and insects. Typical examples for each are listed in Annex A.