本文件规定了一种实时聚合酶链式反应（real-time PCR）方法，用于定性检测来自食物和饲料的山羊特异性DNA。它需要从相关基质中提取足够量的PCR可扩增DNA，并且可以应用于检测源自山羊（Capra hircus）的山羊材料。 靶序列是山羊9号染色体DNA序列(即GenBank登录号NC_030816.1)[1]的部分片段，其以每个单倍体基因组的单拷贝形式存在。所提供的用于该靶标的PCR分析具有每个反应5个拷贝的绝对检测限，在该浓度下具有≥95%的可重复性（LOD95%）。

This document specifies a real-time polymerase chain reaction (real-time PCR) method for the qualitative detection of goat-specific DNA derived from food and feed. It requires the extraction of an adequate amount of PCR amplifiable DNA from the relevant matrix and can be applied to the detection of goat material derived from goat (Capra hircus). The target sequence is a partial fragment of the goat chromosome 9 DNA sequence (i.e. GenBank accession number NC_030816.1)^[1], which is present as a single copy per haploid genome. The provided PCR assay for this target has an absolute limit of detection of five copies per reaction, with ≥ 95 % replicability at this concentration (LOD_{95 %}).