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Standard Test Method for Total Active Biomass in Cooling Tower Waters (Kool Kount Assay; KKA) 冷却塔水中总活性生物量的标准测试方法(Kool Kount Assay; KKA)
发布日期: 2019-12-01
1.1 本试验方法包括测定冷却塔水中的活性生物量,范围为10 2. 至10 8. cfu/mL。这是一种半定量测试方法。 1.2 该试验方法已成功用于试剂水、生理盐水和冷却塔水。用户有责任确保本试验方法对未经试验基质的水的有效性。 1.3 以国际单位制表示的数值应视为标准值。国际单位制后括号中给出的值仅供参考,不被视为标准值。 1.4 本标准并非旨在解决与其使用相关的所有安全问题(如有)。本标准的用户有责任在使用前制定适当的安全、健康和环境实践,并确定监管限制的适用性。 有关具体的危险说明,请参阅第节 9 . 1.5 本国际标准是根据世界贸易组织技术性贸易壁垒(TBT)委员会发布的《关于制定国际标准、指南和建议的原则的决定》中确立的国际公认标准化原则制定的。 ====意义和用途====== 5.1 该试验方法有助于快速测定冷却塔水中的活性生物质浓度。冷却塔的效率直接受到冷却塔水中生物量浓度的影响。随着生物质浓度的增加,生物膜形成,导致塔中的热交换效率降低。目前用于监测冷却塔中生物量浓度的测试需要至少36小时才能在固体琼脂表面上生长微生物进行计数。 在结果可用之前的36小时内,微生物的复制创造了一个不再由生成的数据表示的水环境。及时的测试结果有助于减少杀生物剂的添加,以控制生物量浓度。库尔·库特在数小时内提供数据,以便更精确地控制水中的活性生物量浓度。
1.1 This test method covers the determination of viable active biomass in cooling tower water in the range from 10 2 to 10 8 cfu/mL. It is a semiquantitative test method. 1.2 This test method was used successfully with reagent water, physiologic saline, and cooling tower waters. It is the user’s responsibility to ensure the validity of this test method for waters of untested matrices. 1.3 The values stated in SI units are to be regarded as standard. The values given in parentheses after SI units are provided for information only and are not considered standard. 1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. For specific hazard statements, see Section 9 . 1.5 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee. ====== Significance And Use ====== 5.1 This test method is useful for rapid determination of viable active biomass concentrations in cooling tower waters. The efficiency of cooling towers is directly affected by the concentration of biomass in the cooling tower waters. As biomass concentrations increase, biofilm formation occurs resulting in a decrease in the efficiency of heat exchange in the tower. Current tests for monitoring the biomass concentration in cooling towers require at least 36 h for growth of the microorganisms on a solid agar surface for counting. Replication of microorganisms over the 36-h period before results are available creates an aqueous environment which is no longer represented by the data generated. Timely test results can assist in minimizing biocide addition to control biomass concentrations. Kool Kount provides data within hours to allow for more precise control of active biomass concentrations in the waters.
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归口单位: D19.24
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