1.1 本规程提供了用于检测物体颜色样本中荧光存在的分光光度法。 注1: 由于添加荧光剂（着色剂、增白剂等）通常是材料制造商有意为之，因此通常可以从材料制造商处获得样品中是否存在荧光特性的信息。 1.2 这种做法需要使用分光光度计，该分光光度仪在340的波长范围内照射样本 nm至700 nm，并且允许根据需要改变样品上的照明的光谱分布。 1.3 在上述限制范围内，这种做法的范围是一般性的，而不是具体的文书或材料。 1.4 本标准并非旨在解决与其使用相关的所有安全问题（如有）。本标准的使用者有责任在使用前制定适当的安全、健康和环境实践，并确定监管限制的适用性。 1.5 本国际标准是根据世界贸易组织技术性贸易壁垒委员会发布的《关于制定国际标准、指南和建议的原则的决定》中确立的国际公认的标准化原则制定的。 ===意义和用途====== 4.1 若干标准，包括实践 E991 ， E1164 ，和测试方法 E1331 ， E1348 和 E1349 ，需要存在或不存在样品所表现出的荧光才能正确应用。本规程提供了用于识别材料中荧光存在的分光光度法程序。 4.2 本规程适用于所有物体颜色试样，无论是不透明、半透明还是透明试样，均符合 2.1 半透明试样应通过反射率进行测量，在测量过程中，将标准非荧光背衬材料（通常但不一定是黑色）放置在试样后面。 4.3 这种做法需要使用分光光度计，其中用户可以通过几种方式之一改变样本上照明的光谱分布。照明的修改可以通过在照明源和样本之间插入滤光器，而不干扰来自样本的辐射的检测，或者通过仪器的照明和检测系统的交换，或者通过扫描照明能量和检测输出，如在双单色仪方法中。 4.4 通过比较光谱曲线、色差或测量值之间的ΔY等单参数差异来确认荧光的存在。 注2: 在的版本中 E1247 – 92和更早的时候，荧光的测试是两组光谱透射率或辐射系数（反射系数）相差1 % 在最大差异的波长下的满刻度。 4.5 双向或半球形仪器几何形状可用于此实践。仪器必须能够对样本进行宽带（白光）照射或单色照射和单色检测。 4.6 该实践描述了仅检测荧光存在的方法。它没有解决荧光对任何给定应用的样品比色特性的贡献是显著还是不显著的问题。 用户必须确定荧光对颜色测量的影响的实际意义。

1.1 This practice provides spectrophotometric methods for detecting the presence of fluorescence in object-color specimens. Note 1: Since the addition of fluorescing agents (colorants, whitening agents, etc.) is often intentional by the manufacturer of a material, information on the presence or absence of fluorescent properties in a specimen may often be obtained from the maker of the material. 1.2 This practice requires the use of a spectrophotometer that both irradiates the specimen over the wavelength range from 340 nm to 700 nm and allows the spectral distribution of illumination on the specimen to be altered as desired. 1.3 Within the above limitations, this practice is general in scope rather than specific as to instrument or material. 1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. 1.5 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee. ====== Significance And Use ====== 4.1 Several standards, including Practices E991 , E1164 , and Test Methods E1331 , E1348 and E1349 , require either the presence or absence of fluorescence exhibited by the specimen for correct application. This practice provides spectrophotometric procedures for identifying the presence of fluorescence in materials. 4.2 This practice is applicable to all object-color specimens, whether opaque, translucent, or transparent, meeting the requirements for specimens in the appropriate standards listed in 2.1 . Translucent specimens should be measured by reflectance, with a standard non-fluorescent backing material, usually but not necessarily black, placed behind the specimen during measurement. 4.3 This practice requires the use of a spectrophotometer in which the spectral distribution of the illumination on the specimen can be altered by the user in one of several ways. The modification of the illumination can either be by the insertion of optical filters between the illuminating source and the specimen, without interfering with the detection of the radiation from the specimen, or by interchange of the illuminating and detecting systems of the instrument or by scanning of both the illuminating energy and detection output as in the two-monochromator method. 4.4 The confirmation of the presence of fluorescence is made by the comparison of spectral curves, color difference, or single parameter difference such as ΔY between the measurements. Note 2: In editions of E1247 – 92 and earlier, the test of fluorescence was the two sets of spectral transmittances or radiance factor (reflectance factors) differ by 1 % of full scale at the wavelength of greatest difference. 4.5 Either bidirectional or hemispherical instrument geometry may be used in this practice. The instrument must be capable of providing either broadband (white light) irradiation on the specimen or monochromatic irradiation and monochromatic detection. 4.6 This practice describes methods to detect the presence of fluorescence only. It does not address the issue of whether the fluorescence makes a significant or insignificant contribution to the colorimetric properties of the specimen for any given application. The user must determine the practical significance of the effect of fluorescence on the color measurement.