Microbiology of the food chain — Horizontal method for the immunoenzymatic detection of staphylococcal enterotoxins in foodstuffs
食物链的微生物学 - 食品中葡萄球菌肠毒素免疫酶检测的水平方法
发布日期:
2017-06-01
ISO 19020:2017规定了检测食品中葡萄球菌肠毒素SEA、SEB、SECs、SED和SEE的筛查方法。它包括两个主要步骤:a)提取,然后根据透析原理进行浓缩;b)使用商用检测试剂盒进行免疫酶检测。
ISO 19020:2017适用于人类食用产品中葡萄球菌肠毒素的筛查。
其他葡萄球菌肠毒素,如SEG、SEH、SEI、SER、SES和SET型也可导致疾病。由于缺乏商用检测试剂盒,ISO 19020:2017仅适用于SEA-to-SEE类型,但也可能适用于其他类型的毒素,具体取决于方法的验证。
ISO 19020:2017 specifies a screening method for the detection of staphylococcal enterotoxins SEA, SEB, SECs, SED and SEE in foodstuffs. It consists of two main steps: a) extraction followed by a concentration based on dialysis principle; and b) an immunoenzymatic detection using commercially available detection kits.
ISO 19020:2017 is applicable to the screening of staphylococcal enterotoxins SEA to SEE in products intended for human consumption.
Other staphylococcal enterotoxins such as types SEG, SEH, SEI, SER, SES and SET can also cause illness. Due to the lack of commercially available detection kits, ISO 19020:2017 is applicable only to types SEA to SEE, but may apply to other types of toxins, subject to validation of the method.