本文件描述了一种高通量评估暴露于NPs的3D模型细胞的细胞毒性反应的方法，无需光学干扰。 本文件中的方法旨在用于在细胞培养和生长以及使用3D模型细胞评估NPs细胞毒性方面有能力的生物测试实验室。 该方法适用于由纳米颗粒、纳米粉末、纳米纤维、纳米管和纳米线等纳米物体组成的材料，以及这些材料的聚集体和团聚体。

This document describes a method for high throughput evaluation of cytotoxic response of 3D model cells exposed to NPs without optical interference. The method in this document is intended to be used in biological testing laboratories that are competent in the culture and growth of cells and the evaluation of cytotoxicity of NPs using 3D-model cells. This method applies to materials that consist of nano-objects such as nanoparticles, nanopowders, nanofibres, nanotubes, and nanowires, as well as aggregates and agglomerates of these materials.