警告-本文件的使用可能涉及危险材料、操作和安全 设备本文件并非旨在解决所有相关的安全问题 与它的使用。本文件的使用者有责任建立适当的安全措施 并在使用前确定任何其他限制的适用性。本文件描述了一种测定MIC的参考方法，即肉汤微量稀释法。MIC可以作为临床医生的指南，通过考虑其他因素，如药物药理学、药代动力学或细菌耐药性机制，反映药物在所述试验条件下的活性。 这允许将细菌分类为“易感”（S），“中间”（I）或“抗性”（R）此外，MIC分布可用于定义野生型或非野生型细菌种群。尽管MIC值的临床解释超出了本文件的范围，但某些抗菌剂-细菌组合需要修改基本方法，以便于临床解释。这些修改包括在 本文件的单独附件。有必要比较其他敏感性测试方法（例如。 圆盘扩散或诊断试验装置）进行验证，以确保 可比较且可靠的结果。购买本文件时可获得的所有当前修订均包含在购买本文件中。

WARNING — The use of this document may involve hazardous materials, operations and equipment. This document does not purport to address all of the safety problems associated with its use. It is the responsibility of the user of this document to establish appropriate safety and health practices and to determine the applicability of any other restrictions prior to use.This document describes one reference method, broth micro-dilution, for determination of MICs. The MIC can be a guide for the clinician, and reflects the activity of the drug under the described test conditions, by taking into account other factors, such as drug pharmacology, pharmacokinetics, or bacterial resistance mechanisms. This allows categorisation of bacteria as "susceptible" (S), 'intermediate" (I), or "resistant" (R). In addition, MIC distributions can be used to define wild type or non-wild type bacterial populations. Although clinical interpretation of the MIC value is beyond the scope of this document, modifications of the basic method are required for certain antimicrobial agent - bacteria combinations to facilitate clinical interpretation. These modifications are included in a separate annex of this document. It is necessary to compare other susceptibility testing methods (e.g. disc diffusion or diagnostic test devices) with this reference method for validation, in order to ensure comparable and reliable results.All current amendments available at time of purchase are included with the purchase of this document.