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Journal AWWA - Detecting an Algal Toxin by High-Pressure Liquid Chromatography AWWA杂志-通过高压液相色谱法检测藻类毒素
发布日期: 1982-10-01
本文介绍了一项研究的重点,该研究旨在确定高压液相色谱法(HPLC)和紫外线(UV)检测作为快速筛选技术的适用性,用于分离、鉴定和定量分析anatoxin-a,anatoxin-a是一种由某些水华鱼腥藻菌株产生的神经毒素。正相硅胶柱(含80%的异丙醇己烷溶液)或C18反相柱(含80%的乙腈水溶液)具有良好的分离效果。这项研究还表明了HPLC技术在分离和分析水中不可加工和不易挥发的天然有机化合物方面的适用性。 包括7个参考文献、图表。
This article presents highlights of a study conducted to determine the applicability of high-pressure liquid chromatography (HPLC) with ultraviolet (UV) detection as a rapid screening technique for the isolation,identification, and quantification of anatoxin-a, a neurotoxin produced by certain strains of Anabaena flos-aquea. A normal phase Silica-A column with an 80 percent solution of isopropanol in hexane or a C18 reverse phase column with an 80 percent aqueous solution of acetonitrile gave good separation. This study also showed the applicability of the HPLC technique for isolating and analyzing the nonpurgeable and nonvolatile natural organic compounds in water. Includes 7 references, figures.
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发布单位或类别: 美国-美国给水工程协会
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