本国际标准规定了测定化学品、水和水毒性的半静态方法 废水对淡水鱼Danio rerio胚胎和早期幼体发育阶段的影响 （汉密尔顿·布坎南），真骨鱼类，鲤科（俗名斑马鱼）。如有必要，本决定可以 包括使用Danio rerio进行急性毒性试验，以根据 ISO 7346-1、ISO 7346-2或ISO 7346-3。 如果对试验进行适当修改，该方法也适用于斑马鱼以外的淡水鱼 创造条件，特别是关于每种鱼类生物量的温度和体积。

This International Standard specifies a semi-static method for determination of toxicity of chemicals, waters and wastewaters to embryos and early larval developmental stages of a species of freshwater fish, Danio rerio (Hamilton-Buchanan), Teleostei, Cyprinidae (common name zebrafish). Where necessary this determination may include an acute toxicity test using Danio rerio to determine the 96-h LC50 for zebrafish in accordance with ISO 7346-1, ISO 7346-2 or ISO 7346-3. The method is also applicable to freshwater fish other than zebrafish if appropriate modifications of the test conditions are made, particularly with regard to the temperature and volume per fish biomass.