本文档是EN 13130的一部分，规定了以下两种方法。 方法A是用于测定单体1,2-DHB， 1,3-DHB和1,4-DHB在食品模拟水中，3％w/v乙酸水溶液，15％v/v含水乙醇和橄榄油。单体测定的水平以千克单位/千克食物模拟物表示。该方法适合于在食品模拟物中以0.15mg/kg的最低水平定量测定1,2-DHB和1,3-DHB，并且1,4-DHB在最低水平为0,10 mg/kg食品模拟。 方法B是用于测定食品模拟物水，3％w/v乙酸水溶液，15％v/v含水乙醇和橄榄油中的DHBphen和DHBiph的分析程序。该方法适用于在食品模拟物中分别以0.2mg/kg和0.25mg/kg的最低水平定量测定DHBphen或DHBiph。 注意方法还应适用于其他水性食品模拟物以及其他脂肪食品模拟物。向日葵油，玉米油或合成甘油三酯的混合物

This document, part of EN 13130, specifies two methods as follows. Method A is an analytical procedure for the determination of the monomers 1,2-DHB, 1,3-DHB and 1,4-DHB in the food simulants water, 3 % w/v aqueous acetic acid, 15 % v/v aqueous ethanol and olive oil. The levels of monomer determined are expressed in milligrams of monomer per kilogram of food simulant. The method is appropriate for the quantitative determination of 1,2-DHB and 1,3-DHB at a minimum level of 0,15 mg/kg in food simulants and, of 1,4-DHB at a minimum level of 0,10 mg/kg in food simulants. Method B is an analytical procedure for the determination of DHBphen and DHBiph in the food simulants water, 3 % w/v aqueous acetic acid, 15 % v/v aqueous ethanol and olive oil. The method is appropriate for the quantitative determination of DHBphen or DHBiph at minimum levels of 0,2 mg/kg and 0,25 mg/kg, respectively, in food simulants. NOTE The methods should also be applicable to other aqueous food simulants as well as to the other fatty food simulants e.g. sunflower oil, corn oil or a mixture of synthetic triglycerides.