1.1 这些测试方法通过提供在两种琼脂基质（一种促进微生物生长，另一种不促进微生物生长）上测试薄膜的方法，测试粘合薄膜抑制或支持在琼脂平板上生长的选定真菌物种生长的能力。 1.2 这些测试方法并不适用于所有粘合剂。由于与某些介质成分发生不可逆反应，这些测试方法可能无法证明某些杀生物剂的活性。 注1: 例如，季铵化合物被琼脂灭活。 1.3 包括一种用于低粘度粘合剂的测试方法，以及一种用于胶泥型粘合剂的替代方法。 1.4 以国际单位制表示的数值应视为标准。括号中给出的值仅供参考。 1.5 本标准并非旨在解决与其使用相关的所有安全问题（如有）。 本标准的使用者有责任在使用前制定适当的安全、健康和环境实践，并确定监管限制的适用性。 这些测试方法旨在供受过正确微生物技术培训的人员使用。第节中给出了具体的预防说明 7. 和在 14.3.2 . 1.6 本国际标准是根据世界贸易组织技术性贸易壁垒委员会发布的《关于制定国际标准、指南和建议的原则的决定》中确立的国际公认的标准化原则制定的。 ====意义和用途====== 4.1 这些测试方法旨在确定粘合膜对生物降解的敏感性，以及粘合剂是否会携带足够的抗- 防止真菌生长的真菌特性经常出现在胶粘设备、粘附物或所用粘合剂上。 4.2 马铃薯葡萄糖琼脂（PDA）为真菌的生长提供了完整的培养基，而矿物盐琼脂（MSA）缺乏碳水化合物来源，提供了不太有利的培养基。PDA的使用测试了粘合膜抵抗真菌在其表面上生长的能力以及排斥真菌在相邻琼脂表面上大量生长的能力。MSA的使用主要测试粘合膜抵抗表面真菌生长的能力。使用时，由于相邻琼脂上的真菌生长很少，琼脂上的生长被错误解读为来自粘合膜的可能性降低。 注2: 此处给出的使用MSA的方法基于实践 第21组 ，适用于与粘合剂一起使用。 MSA的使用要求见 10.2 ，中规定了一种混合真菌 8.2 接种物。 4.3 使用本方法中给出的程序时获得的结果仅适用于用于测试的物种。第节中列出的试验物种 8. 实验室经常使用它们来测试抗真菌特性，但它们并不是唯一可以使用的。选择要测试的真菌物种需要测试实验室或要求测试的一方做出知情判断。当使用从受污染的粘合剂中分离出来的物种作为测试物种时，这些方法尤其有用（见第 8. )以帮助选择更有效的杀菌剂。 4.4 由于某些杀生物剂作为某些粘合剂的组分所处的化学和热环境，它们在储存过程中的功效可能会发生变化。 这些测试方法不适用于在浸水条件下确定真菌污染对粘合剂的影响，因为它们的设计并没有涵盖水溶性杀生物剂从粘合线中浸出的可能性。 4.5 这些测试方法取决于活微生物在一组已报道的条件下的生理作用。通过将结果与已知耐药性的同时运行对照进行比较，可以得出关于测试粘合剂对真菌攻击的耐药性的结论。看见 X5.2毫米 关于测试重复性的声明。

1.1 These test methods test the ability of adhesive films to inhibit or support the growth of selected fungal species growing on agar plates by providing means of testing the films on two agar substrates, one which promotes microbial growth, and one which does not. 1.2 These test methods are not appropriate for all adhesives. The activity of certain biocides may not be demonstrated by these test methods as a result of irreversible reaction with some of the medium constituents. Note 1: As an example, quaternary ammonium compounds are inactivated by agar. 1.3 A test method is included for use with low-viscosity adhesives along with an alternative method for use with mastic-type adhesives. 1.4 The values stated in SI units are to be regarded as the standard. The values given in parentheses are for information only. 1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. These test methods are designed to be used by persons trained in correct microbiological techniques. Specific precautionary statements are given in Section 7 and in 14.3.2 . 1.6 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee. ====== Significance And Use ====== 4.1 These test methods are designed to be used to determine the susceptibility of the adhesive film to biodegradation and whether the adhesive will carry into the bond line sufficient anti-fungal properties to prevent growth of fungi frequently present on the gluing equipment, on adherends, or in the adhesive as applied. 4.2 Potato dextrose agar (PDA) provides a complete medium for the growth of fungi, while mineral salts agar (MSA) lacks a carbohydrate source and provides a less favorable medium. Use of PDA tests the adhesive film for its ability to resist the growth of fungi on its surface as well as its ability to repel a copious growth of fungi on the adjacent agar surface. Use of MSA tests the adhesive film primarily for its ability to resist the growth of fungi on its surface. When it is used, there is a reduced possibility that the growth from the agar will be mis-read as coming from the adhesive film, since fungal growth on the adjacent agar will be scant. Note 2: The method given here using the MSA is based on Practice G21 , adapted to be used with adhesives. Requirements for the use of the MSA are described in 10.2 , and a mixed species of fungi is prescribed in 8.2 for the inoculum. 4.3 The results obtained when using the procedures given in this method apply only to the species used for the testing. The test species listed in Section 8 are frequently used by laboratories to test for antifungal properties, but they are not the only ones which could be used. Selection of the fungal species to test against requires informed judgment by the testing laboratory or by the party requesting the tests. These methods are especially useful when species that have been isolated from contaminated adhesives are used as the test species (see Section 8 ) to aid in the selection of more effective fungicides. 4.4 The efficacy of some biocides may change in storage due to the chemical and thermal environment to which they are subjected as components of certain adhesives. These test methods are not appropriate for determining the effect of fungal contamination on adhesives under water-soaking conditions, because they are not designed to cover the possibility of water-soluble biocides leaching out of the bond line. 4.5 These test methods are dependent upon the physiological action of living microorganisms under a reported set of conditions. Conclusions about the resistance of the test adhesive to fungal attack can be drawn by comparing the results to simultaneously run controls of known resistance. See X5.2 for statements regarding test repeatability.